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PE/Cyanine7 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-PE/Cyanine7
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Product Citations
publications
PECyanine7_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with PE/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (PE/Cyanine7) Streptavidin (filled histogram).
  • PECyanine7_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with PE/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (PE/Cyanine7) Streptavidin (filled histogram).
See PE/Cyanine7 spectral data
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405206 100 µg 165€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine7 by 488 nm laser light induces a light emission maximum of 775 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PE/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-PE/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine7 conjugation.  For immunofluorescent staining, the recommended use of this reagent is =0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine7 is 360 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. Kamimura D, et al. 2006. J. Immunol. 177:306.
  2. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  3. Anderson SM, et al. 2007. J. Exp. Med. doi:10.1084/jem.20062571. PubMed
  4. Rigolio R, et al. 2008. J Neuroimmunol. 199:67. PubMed
  5. Chappaz S, et al. 2010. J. Immunol. 184:3562. PubMed
  6. Weber SE, et al. 2011. J. Immunol. 186:432. PubMed
  7. Chamoto, K., et al. 2011. Am J Respir Cel Mol Biol. PubMed
  8. Ahuja, A., et al. 2011. J. Immunol. 187:3888. PubMed
  9. Chamoto K, et al. 2012. Am J Respir Cell Mol Biol. 46:283. PubMed
  10. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  11. Nakamura M, et al. 2014. Mult Scler. PubMed
  12. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  13. Shaw OM, et al. 2014. Rheumatology. PubMed
  14. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  15. Yasunaga M, et al. 2014. Sci Rep. 4:4852. PubMed
  16. Nakamura M, et al. 2014. Mult Scler. 20:1371. PubMed
  17. Shaw OM, et al. 2014. Rheumatology. 53:1901. PubMed
  18. Tang AH, et al. 2014. EMBO J. 33:2782. PubMed
Product Citations
  1. Li Y, et al. 2020. Nat Commun. 2.350694444. PubMed
  2. Hu XF, et al. 2021. Theranostics. 3796:11. PubMed
  3. Geng G, et al. 2021. Elife. 10: . PubMed
  4. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  5. Ahuja A, et al. 2011. J Immunol. 187:3888. PubMed
  6. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  7. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  8. Afroj T, et al. 2021. J Immunol. 206:1204. PubMed
  9. Zhou S, et al. 2020. Cell Res. 30:1063. PubMed
  10. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  11. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  12. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  13. Finke S 2010. J Immunol. 184:3562. PubMed
  14. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  15. Zheng H, et al. 2017. Sci Rep. 7:37. PubMed
  16. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  17. Anderson S, et al. 2007. J Exp Med. 204:2103. PubMed
  18. Tang D, et al. 2020. Br J Haematol. 191:906. PubMed
  19. Khateb M, et al. 2022. Cell Rep. 40:111219. PubMed
  20. Han YH, et al. 2021. Science. 373:. PubMed
  21. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  22. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  23. Zou T, et al. 2010. J Immunol. 185:2790. PubMed
  24. Schenk R, et al. 2017. Cell Death Differ. 10.1038/cdd.2016.156. PubMed
  25. Chamoto K, et al. 2012. Am J Respir Cell Mol Biol. 46:283. PubMed
  26. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  27. Gressler AE, et al. 2022. Front Immunol. 13:896255. PubMed
  28. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  29. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  30. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  31. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
  32. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  33. Trinh T, et al. 2020. Leukemia. . PubMed
  34. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  35. Schmid M, et al. 2016. PLoS One. 12: 1005676. PubMed
  36. Sebastian M, et al. 2016. J Immunol. 196: 144 - 155. PubMed
  37. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  38. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  39. Tarke A, et al. 2022. Cell. 185:847. PubMed
  40. Matsumura M 2014. Sci Rep. 4:4852. PubMed
  41. Rando A 2014. EMBO J. 33:2782. PubMed
  42. Cassotta A, et al. 2020. J Exp Med. 217:00:00. PubMed
  43. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
  44. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  45. Mueller A, et al. 2016. Nature. 540:276-279. PubMed
  46. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  47. Shaw O, et al. 2014. Rheumatology. 53:1901. PubMed
  48. Zhi Z, et al. 2021. Cell Death Differ. Online ahead of print.. PubMed
  49. Dan JM, et al. 2021. Science. :371. PubMed
  50. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  51. Nakamura M, et al. 2014. Mult Scler. 20:1371. PubMed
  52. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
  53. Zeng W, et al. 2022. Nat Commun. 13:947. PubMed
  54. Rigolio R, et al. 2008. J Neuroimmunol. 199:67. PubMed
  55. Kato Y, et al. 2015. J Immunol. 195: 1006-1014. PubMed
  56. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  57. Werner A, et al. 2021. iScience. 24:103076. PubMed
  58. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  59. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  60. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  61. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  62. Getahun A, et al. 2016. J Exp Med. 213: 751 - 769. PubMed
  63. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
  64. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  65. Tichy ED, et al. 2021. STAR Protoc. 2:100830. PubMed
  66. Tichy ED, et al. 2021. Cell Rep. 35:109098. PubMed
  67. Spath S, et al. 2022. iScience. 25:104998. PubMed
  68. Weber S, et al. 2011. J Immunol. 186:432. PubMed
  69. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed

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Go To Top Version: 5    Revision Date: 04/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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