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PE anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_PE_062310
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 6D5_PE_062310
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 6D5_PE_CD19_Antibody_042922
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (solid line) or rat IgG2a, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
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115507 50 µg 36€
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115508 200 µg 108€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
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  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
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  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
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  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
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  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
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  12. Silver AC, et al. 2018. Heliyon. 4:e00579. PubMed
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  16. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  17. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  18. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  19. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
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  22. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  23. Yamaguchi T, et al. 2010. Genes Dev. 24:455. PubMed
  24. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  25. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  26. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  27. Hu EY, et al. 2021. Blood Adv. 5:3152. PubMed
  28. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
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  31. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
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  33. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  34. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
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  36. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
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  38. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
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  41. Cho K, et al. 2021. iScience. 24:103117. PubMed
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  57. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  58. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  59. Muenchow L, et al. 2014. Immunol Lett. 160:113. PubMed
  60. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  61. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
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  64. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  65. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  66. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  67. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  68. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  69. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  70. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  71. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  72. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  73. Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed
  74. Aulova KS, et al. 2022. Molecules. 27:. PubMed
  75. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  76. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  77. Aalipour A, et al. 2020. Mol Ther Oncolytics. 17:232. PubMed
  78. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  79. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
  80. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  81. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  82. Schubert M, et al. 2021. Cancers (Basel). 13: . PubMed
  83. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  84. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  85. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  86. Wali S, et al. 2020. Am J Respir Cell Mol Biol. 758:63. PubMed
  87. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  88. Syres K, et al. 2009. Blood. 114:2452. PubMed
  89. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  90. Gawish R, et al. 2022. Elife. 11:. PubMed
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RRID
AB_313642 (BioLegend Cat. No. 115507)
AB_313643 (BioLegend Cat. No. 115508)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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