Loading...

FITC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Fluorescein Isothiocyanate, SAv-FITC
Ave. Rating
Submit a Review
Product Citations
publications
SAV_FITC_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
  • SAV_FITC_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
405201 100 µg 34€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
405202 500 µg 95€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the FITC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

Application References
  1. Schmutz S, et al. 2009. J. Immunol. 183:2217. PubMed
  2. Cho HY, et al. 2009. Immunol Lett. 127:39. PubMed
  3. de Wispelaere D, et al. 2012. J. Virol. 86:7072. PubMed
  4. Fukuoka A, et al. 2013. Int Immunol. PubMed
  5. Giltiay NV, et al. 2013. J Exp Med. 25:373. PubMed
  6. Lee JH, et al. 2014. J Rheumatol. 41:2425. PubMed
Product Citations
  1. Georgiadis C, et al. 2021. Leukemia. . PubMed
  2. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  3. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  4. Sasaki N, Itakura Y, Toyoda M 2017. Stem Cell Res Ther. 10.1186/s13287-017-0534-1. PubMed
  5. Suarez-Calvet X, et al. 2021. FASEB J. 35:e21819. PubMed
  6. Zaitouna AJ, et al. 2020. Bio Protoc. 10:e3655. PubMed
  7. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
  8. Sánchez-Salguero E, et al. 2021. Front Immunol. 12:712130. PubMed
  9. Hu Y, et al. 2022. Nat Commun. 13:5313. PubMed
  10. Yang M 2012. J Virol. 86:7072. PubMed
  11. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  12. Li S, et al. 2016. PLoS One. 11: 0163893. PubMed
  13. Lee J, et al. 2014. J Rheumatol. 41:2425. PubMed
  14. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  15. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  16. Vigeland C, et al. 2016. PLoS One. 11: 0163288. PubMed
  17. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  18. Zhang Y, et al. 2019. JCI Insight. 4:2. PubMed
  19. Yan R, et al. 2021. Cell Death Dis. 12:955. PubMed
  20. Schmutz S, et al. 2009. J Immunol. 183:2217. PubMed
  21. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  22. Cheng LS, et al. 2022. J Transl Med. 20:415. PubMed
  23. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  24. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  25. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  26. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
  27. Bülow JM, et al. 2022. Front Endocrinol (Lausanne). 13:1016057. PubMed
  28. Oda Y, et al. 2021. Sci Adv. 7:eabj6895. PubMed
  29. Li X, et al. 2021. Oncol Lett. 22:788. PubMed
  30. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  31. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
  32. Cho H, et al. 2009. Immunol Lett. 127:39. PubMed
  33. Hensel JA, et al. 2019. Mol Ther Oncolytics. 12:41. PubMed
  34. Mödinger Y, et al. 2018. J Cell Mol Med. 22:6002. PubMed
  35. Hamester F, et al. 2022. Cells. 11:. PubMed
  36. Lin Y, et al. 2012. PLoS One. 7:e30507. PubMed
  37. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  38. Giltiay N, et al. 2013. J Exp Med. 210:2773. PubMed
  39. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  40. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  41. Laurin M, et al. 2019. Elife. 8. PubMed
  42. Sturmlechner I, et al. 2021. Science. 374:eabb3420. PubMed
  43. Zhu Z, et al. 2017. Int J Biochem Cell Biol. 10.1016/j.biocel.2017.04.003. PubMed
  44. Lindsay RS, et al. 2021. J Exp Med. 218:. PubMed
  45. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
  46. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
Go To Top Version: 5    Revision Date: 06/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account