Brilliant Violet 605™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
M1slash70_BV605_020212
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 605™. Data shown was gated on myeloid cell population.
  • M1slash70_BV605_020212
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 605™. Data shown was gated on myeloid cell population.
Compare all formats See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
101237 125 µL 168€
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101257 50 µg 206€
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  3. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  4. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  5. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  6. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  7. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  8. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  9. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  10. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  11. Boal-Carvalho I, et al. 2020. EMBO Rep. 21:e50421. PubMed
  12. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  13. Rosenheinrich M, et al. 2015. PLoS One. 10: 0136290. PubMed
  14. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  15. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  16. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  17. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  18. Frank AC, et al. 2021. Theranostics. 11:7570. PubMed
  19. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  20. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  21. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  22. Hu J, et al. 2021. Sci Adv. 7:. PubMed
  23. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  24. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  25. Bauer KM, et al. 2022. JCI Insight. 7:. PubMed
  26. Morris MG, et al. 2020. Comp Med. 70:336. PubMed
  27. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  28. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  29. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  30. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  31. Huard A, et al. 2021. J Immunol. 206:1058. PubMed
  32. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  33. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  34. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  35. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  36. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  37. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  38. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  39. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  40. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  41. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  42. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  43. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  44. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  45. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  46. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  47. Iannello A, et al. 2013. J Exp Med. 210:2057. PubMed
  48. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  49. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  50. Dar HH, et al. 2022. JCI Insight. 7:. PubMed
  51. Li Y, et al. 2022. J Neuroinflammation. 19:261. PubMed
  52. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  53. McBride KM, et al. 2019. Front Oncol. 9:517. PubMed
  54. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  55. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  56. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  57. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  58. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  59. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  60. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  61. Yang H, et al. 2019. J Neuroinflammation. 16:169. PubMed
  62. Sun T, et al. 2017. Mucosal Immunol. 0.954861111. PubMed
  63. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  64. Yang C, et al. 2022. Research (Wash D C). 2022:9768687. PubMed
  65. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  66. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  67. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  68. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  69. Dudeck J, et al. 2019. J Allergy Clin Immunol. 143:1849. PubMed
  70. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  71. Wang YT, et al. 2020. Nat Microbiol. 0.397222222. PubMed
  72. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  73. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  74. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  75. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  76. Hamaidi I, et al. 2020. Cell Metabolism. 32(3):420-436.e12. PubMed
  77. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  78. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  79. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  80. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  81. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  82. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  83. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  84. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  85. Shi H, et al. 2015. Nat Immunol. . PubMed
  86. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  87. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  88. Nam J, et al. 2021. Adv Ther (Weinh). 4:. PubMed
  89. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  90. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  91. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  92. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  93. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  94. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  95. Melhem NJ, et al. 2021. Circulation. 143:566. PubMed
  96. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  97. Crichton M, et al. 2016. Sci Rep. 6:27217. PubMed
  98. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  99. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  100. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  101. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  102. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  103. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  104. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  105. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  106. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  107. Wang T, et al. 2015. Proc Natl Acad Sci U S A. 112:440. PubMed
  108. Sparber F et al. 2019. British journal of pharmacology. 176(11):1728-1744 . PubMed
  109. Bretou M, et al. 2017. Sci Immunol. 2:16. PubMed
  110. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
  111. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  112. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
  113. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  114. C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
RRID
AB_11126744 (BioLegend Cat. No. 101237)
AB_2565431 (BioLegend Cat. No. 101257)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC,IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 806 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 780 anti-mouse/human CD11b M1/70 FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
Spark PLUS UV™ 395 anti-mouse/human CD11b M1/70 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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