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Biotin anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_Biotin_090507
C57BL/6 mouse splenocytes stained with biotinylated 30-F11, followed by Sav-PE
  • 30-F11_Biotin_090507
    C57BL/6 mouse splenocytes stained with biotinylated 30-F11, followed by Sav-PE
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103103 50 µg 44€
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103104 500 µg 194€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yen W, et al. 2012. Clin Cancer Res. 18:5374. PubMed
  2. Dell'Aringa M, et al. 2018. Mucosal Immunol. 1.207638889. PubMed
  3. Tarban N, et al. 2022. Cells. 11:. PubMed
  4. Fujimori S, et al. 2022. Elife. 11:. PubMed
  5. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  6. Kaminitz A, et al. 2013. Int Immunol. 25:485. PubMed
  7. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  8. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
  9. Xie T, et al. 2021. Sci Adv. 7: . PubMed
  10. Muhsin-Sharafaldine M, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.03.138. PubMed
  11. Wagner F, et al. 2016. J Bone Joint Surg Am. 98: 916 - 925. PubMed
  12. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  13. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  14. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  15. Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed
  16. Ibrahim AM, et al. 2021. Breast Cancer Res. 23:90. PubMed
  17. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  18. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  19. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  20. Howard E, et al. 2020. J Allergy Clin Immunol. . PubMed
  21. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  22. Nevius E, et al. 2015. J Exp Med. 212: 1931 - 1946. PubMed
  23. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  24. Wang Z, et al. 2022. Cell Mol Immunol. 19:210. PubMed
  25. Shen Z, et al. 2021. iScience. 24:103014. PubMed
  26. Lv J, et al. 2021. Cell Discov. 7:24. PubMed
  27. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
  28. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  29. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  30. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
  31. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
  32. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
  33. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  34. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  35. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  36. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  37. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
  38. Pual VM, et al. 2019. PLOS Biology. 17(10):e3000492. PubMed
  39. Barnes SE, et al. 2021. J Neuroinflammation. 18:199. PubMed
  40. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  41. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
  42. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  43. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  44. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  45. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  46. Budai Z, et al. 2021. Cells. 10:. PubMed
  47. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
  48. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  49. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  50. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  51. Seong J, et al. 2018. Development. 145:14. PubMed
  52. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
RRID
AB_312968 (BioLegend Cat. No. 103103)
AB_312969 (BioLegend Cat. No. 103104)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV™ 395 anti-mouse CD45 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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