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APC anti-human IFN-γ Antibody

Pricing & Availability
Clone
4S.B3 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
4S.B3_APC_IFNg_Antibody_FC_1_010714
PMA+ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, then fixed with Fixation Buffer (Cat# 420801), and permeabilized with Permeabilization Wash Buffer (Cat# 421002). Cells were then stained with IFN-γ (clone 4S.B3) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 4S.B3_APC_IFNg_Antibody_FC_1_010714
    PMA+ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, then fixed with Fixation Buffer (Cat# 420801), and permeabilized with Permeabilization Wash Buffer (Cat# 421002). Cells were then stained with IFN-γ (clone 4S.B3) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 4S.B3_APC_IFNg_Antibody_FC_2_010714
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502511 25 tests 96€
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502512 100 tests 228€
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  2. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  3. Goetzmann J, et al. 2014. Proc Natl Acad Sci U S A. 111:8873. PubMed
  4. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  5. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  6. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  7. Chandran A, et al. 2022. Cell Rep Med. 3:100557. PubMed
  8. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  9. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
  10. Pandey A, et al. 2020. Nat Commun. 3.392361111. PubMed
  11. Norian L, et al. 2009. Cancer Res. 69:3086. PubMed
  12. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  13. Longhi M, et al. 2014. PLoS One. 9:87956. PubMed
  14. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  15. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  16. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  17. Cai J, et al. 2021. eLife. 10:00. PubMed
  18. Baban B, et al. 2021. JCI Insight. 6:00. PubMed
  19. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  20. Port JR, et al. 2020. J Virol. 94:. PubMed
  21. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  22. Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed
  23. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  24. Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed
  25. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  26. Zhang X, et al. 2017. J Immunol. 10.4049/jimmunol.1602183. PubMed
  27. Du Q, et al. 2018. J Immunol. 201:533. PubMed
  28. Ge C, et al. 2020. Cell Reports. 29(13):4236-4244.e3.. PubMed
  29. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  30. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  31. O'Donoghue GP, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  32. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  33. Boutboul D, et al. 2018. J Clin Invest. 128:3071. PubMed
  34. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  35. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  36. Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
  37. Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
  38. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  39. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  40. Tian X, et al. 2015. J Immunol. 194:3873. PubMed
  41. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  42. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  43. Tesch S, et al. 2020. Sci Rep. 10:21312. PubMed
RRID
AB_315236 (BioLegend Cat. No. 502511)
AB_315237 (BioLegend Cat. No. 502512)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 2    Revision Date: 01/07/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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