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PE/Cyanine7 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_PECy7_111407
Human peripheral blood lymphocytes stained with UCHT1 PE/Cyanine7 and overlayed with isotype control
  • UCHT1_PECy7_111407
    Human peripheral blood lymphocytes stained with UCHT1 PE/Cyanine7 and overlayed with isotype control
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
300419 25 tests £98
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300420 100 tests £191
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  2. Jiang L, et al. 2021. Int J Biol Sci. 17:4377. PubMed
  3. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  4. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  5. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  6. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  7. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  8. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  9. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  10. Goel G, et al. 2019. Sci Adv. 5:eaaw7756. PubMed
  11. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  12. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  13. Vijayakumar B, et al. 2022. Immunity. . PubMed
  14. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  15. Ridley ML, et al. 2020. J Immunol. 204:2940. PubMed
  16. Todorova D, et al. 2020. EBioMedicine. 62:103120. PubMed
  17. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  18. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  19. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  20. Ramirez NP, et al. 2022. Nat Commun. 13:1109. PubMed
  21. Seumois G, et al. 2016. J Immunol. 197: 655 - 664. PubMed
  22. Leone D, et al. 2016. PLoS One. 11: 0151674. PubMed
  23. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  24. Taraldsrud E, et al. 2017. Journal of Autoimmunity. 10.1016/j.jaut.2017.04.004. PubMed
  25. Usero L, et al. 2016. Diabetes. 65: 2356 - 2366. PubMed
  26. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  27. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  28. Cartellieri M, et al. 2014. PLoS One. 9:93745. PubMed
  29. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  30. Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
  31. Alonso R, et al. 2022. Blood Adv. :. PubMed
  32. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  33. Vela M, et al. 2019. Sci Rep. 9:18792. PubMed
  34. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  35. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  36. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  37. Moore N, et al. 2019. Sci Rep. 9:15101. PubMed
  38. Andreano E, et al. 2021. Nature. Online ahead of print. PubMed
  39. Comte D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9321 - 9326. PubMed
  40. Rosskopf S, et al. 2018. Oncotarget. 9:17608. PubMed
  41. Leone DA, et al. 2017. J Immunol. 199:531. PubMed
  42. Cartellieri M, et al. 2016. Blood Cancer J. 6:e458. PubMed
  43. Iio K, et al. 2019. Sci Rep. 9:813. PubMed
  44. Román-Rodriguez FJ, et al. 2019. Cell Stem Cell. 25:607. PubMed
  45. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  46. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  47. Gustafson CE, et al. 2020. JCI Insight. 5:. PubMed
  48. Ishigaki K, et al. 2015. Sci Rep. 5: 12937. PubMed
  49. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  50. Lo Presti E, et al. 2020. J Leukoc Biol. 108:749. PubMed
  51. Cruz-Lorenzo E, et al. 2022. Viruses. 14:. PubMed
  52. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  53. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  54. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  55. Andreano E, et al. 2021. Cell. 184(7):1821-1835.e16. PubMed
  56. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  57. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_439780 (BioLegend Cat. No. 300419)
AB_439781 (BioLegend Cat. No. 300420)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

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Description Clone Applications
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FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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