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Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
400543 100 µg £72
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400544 1 mg £102
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400565 5 mg £369
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400566 25 mg £964
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400573 50 mg £1611
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400574 100 mg £2314
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this rat IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  2. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  3. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  4. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  5. Handke J, et al. 2021. Front Cardiovasc Med. 8:682458. PubMed
  6. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  7. Mbofung R, et al. 2017. Nat Commun. 10.1038/s41467-017-00449-z. PubMed
  8. Nabar NR, et al. 2022. Autophagy. 18:204. PubMed
  9. Bacigaluppi M, et al. 2016. J Neurosci. 36: 10529 - 10544. PubMed
  10. Vito A, et al. 2021. Commun Biol. 4:859. PubMed
  11. Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed
  12. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  13. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  14. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
  15. Kugel CH, et al. 2018. Clin Cancer Res. 24:5347. PubMed
  16. Obert E, et al. 2022. Heliyon. 8:e11359. PubMed
  17. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  18. Walker MT, et al. 2018. J Allergy Clin Immunol. 141:1711. PubMed
  19. Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
  20. Bok I, et al. 2022. JID Innov. 2:100076. PubMed
  21. Verma M, et al. 2021. J Exp Med. 218:. PubMed
  22. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
RRID
AB_11148951 (BioLegend Cat. No. 400543)
AB_11147167 (BioLegend Cat. No. 400544)

Antigen Details

Gene ID
NA

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Biotin Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
FITC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA,ChIP
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Pacific Blue™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl RTK2758 ICC
GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl RTK2758 SB
PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Go To Top Version: 4    Revision Date: 06/06/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2a, κ Isotype Ctrl

  • Biotin Rat IgG2a, κ Isotype Ctrl

  • FITC Rat IgG2a, κ Isotype Ctrl

  • PE Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl

  • Purified Rat IgG2a, κ Isotype Ctrl

    RTK2758_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl

  • PerCP Rat IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl

  • Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl

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