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Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
A17200C (See other available formats)
Regulatory Status
RUO
Other Names
T-Cell Immunoreceptor with Ig and ITIM domains, T cell Ig, Immunoglobulin Domain-Containing Protein 9, V-Set, Transmembrane Domain-Containing Protein 3, WUCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A17200C_ULEAF_TIGIT_Antibody_08042022
Recombinant biotinylated mouse TIGIT (Black Circles) binds to immobilized recombinant mouse CD155/PVR. Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3) Antibody, clone A17200C (Purple squares) inhibits the binding in a dose-dependent manner whereas the isotype control (Cat. No. 400562, not shown) does not have the effect. This antibody blocks the binding of 0.04 µg/mL biotinylated recombinant mouse TIGIT to 1 µg/mL of immobilized recombinant mouse CD155 with an ND50 = 0.01 - 0.06 µg/mL.
  • A17200C_ULEAF_TIGIT_Antibody_08042022
    Recombinant biotinylated mouse TIGIT (Black Circles) binds to immobilized recombinant mouse CD155/PVR. Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3) Antibody, clone A17200C (Purple squares) inhibits the binding in a dose-dependent manner whereas the isotype control (Cat. No. 400562, not shown) does not have the effect. This antibody blocks the binding of 0.04 µg/mL biotinylated recombinant mouse TIGIT to 1 µg/mL of immobilized recombinant mouse CD155 with an ND50 = 0.01 - 0.06 µg/mL.
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622203 100 µg £185
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622204 1 mg £490
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Description

TIGIT (T cell immunoreceptor with Ig and ITIM domains precursor) contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif. TIGIT is an inhibitory receptor expressed on the surface of natural killer (NK) cells. TIGIT recognizes nectin and nectin-like adhesion molecules and thus plays a critical role in the innate immune response to malignant transformation. TIGIT in conjunction with CD226 forms a pathway that has prominent similarities to the costimulatory CD28/CTLA-4 pathway. CD226 and TIGIT bind the same set of ligands (CD155 and CD112) and CD226 is a positive regulator of T cell responses, while TIGIT inhibits them. Knockdown of TIGIT expression in human CD4+ T cells results in an increase of both T-bet and IFN-γ mRNA and protein expression. These effects are overcome by blocking CD226 signaling indicating that TIGIT exerts immunosuppressive effects by competing with CD226 for the same ligand, CD155. The binding of CD155 to TIGIT on human dendritic cells enhances the production of interleukin 10 and diminishes the production of interleukin 12p40. TIGIT shows an inhibitory effect on cell-signaling pathway in murine models of experimental autoimmune encephalomyelitis (EAE). Loss of TIGIT expression in mice results in hyperproliferative T cell responses and increased susceptibility to EAE.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIGIT Recombinant Protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between mCD155 / PVR and mTIGIT. The ND50 range: 0.01 – 0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Koda Y, et al. 2021. Nat Commun. 12:4474. PubMed
RRID
AB_2814472 (BioLegend Cat. No. 622203)
AB_2814473 (BioLegend Cat. No. 622204)

Antigen Details

Structure
Homodimer
Distribution

Human follicular B helper T cells (TFH), regulatory, memory and activated T cells, NK cells.

Function
Immunosuppressive effects by binding CD155 and modulating cytokine production by DC. Inhibits NK cell cytotoxicity. Inhibits T cell functions by competing with CD226. Tregs TIGIT+ cells suppress proinflammatory Th1 and Th17 cells, but not Th2 cell responses.
Interaction
Dendritic cells, monocytes
Ligand/Receptor
Poliovirus receptor (PVR/CD155) and nectin-2 (PRR-2/CD112)
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Yu X, et al. 2009. Nat. Immunol. 10:48.
  2. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. U S A. 106:17858.
  3. Joller N, et al. 2011. J. Immunol. 186:1338.
  4. Tahara-Hanaoka S, et al. 2004. Int. Immunol. 16:533.
  5. Lozano E, et al. 2012. J. Immunol. 188:3869.
  6. Joller N, et al. 2014. Immunity. 40:569.
  7. Deuss FA, et al. 2017. J. Biol. Chem. 292:11413.
Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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