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Ultra-LEAF™ Purified anti-mouse CD8a Recombinant Antibody

Pricing & Availability
Clone
QA17A07 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A07_ULEAF_CD8a_Antibody_1_103020
Depletion of CD8+ T cells from spleen 24h after injection of 250 µg Ultra-LEAF™ purified CD8a recombinant (clone QA17A07) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram). Data shown is gated on T cells, CD8 T cells were labeled with mAb YTS156.7.7 anti-mouse CD8b.
  • QA17A07_ULEAF_CD8a_Antibody_1_103020
    Depletion of CD8+ T cells from spleen 24h after injection of 250 µg Ultra-LEAF™ purified CD8a recombinant (clone QA17A07) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram). Data shown is gated on T cells, CD8 T cells were labeled with mAb YTS156.7.7 anti-mouse CD8b.
  • QA17A07_ULEAF_CD8a_Antibody_2_103020
    C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified CD8a (clone QA17A07) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 PE antibody.
  • QA17A07_ULEAF_CD8a_Antibody_3_103020
    Kinetic of CD8+ T cell depletion after injection of 200ug Ultra-LEAF™ purified CD8a recombinant (clone QA17A07) (blue) or Ultra-LEAF™ purified mouse IgG1, k isotype control (black); both groups were bled at the indicated time points and the percentage of CD8+ T cells from total T cells was determined.
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155011 100 µg £65
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155012 1 mg £102
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse thymus or spleen
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Depletion - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.015 µg per million cells in 100 µL volume. A single i.v. dose of 200 µg of mAb QA17A07 depletes CD8+ T cells from peripheral blood for aproximatelly 30 days. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
RRID
AB_2888825 (BioLegend Cat. No. 155011)
AB_2888825 (BioLegend Cat. No. 155012)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay AN, Brown MH. 1997. Biochem. Soc. Trans. 25:224-8.
  2. Zamoyska R. 1994. Immunity 1:243.
  3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/30/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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