Ultra-LEAF™ Purified anti-mouse CCL20 (MIP-3α) Antibody

Pricing & Availability
Clone
A16054H (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage inflammatory protein-3α (MIP-3α)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A16054H_ULEAF_CCL20_MIP-3alpha_Antibody_080321.png
Recombinant mouse CCL20 (MIP-3α) (Cat. No. 582306) (black circles) induces the chemotaxis of mouse Baf3-hCCR6 transfectant chemoattractant cells. Ultra-LEAF™ purified anti- mouse CCL20 (MIP-3α) antibody (clone A16054H) (purple squares) neutralizes the chemotaxis of recombinant mouse CCL20 (MIP-3α) at 0.3 ng/mL on mouse Baf3-hCCR6 cells in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.08 - 0.4 µg/mL.
  • A16054H_ULEAF_CCL20_MIP-3alpha_Antibody_080321.png
    Recombinant mouse CCL20 (MIP-3α) (Cat. No. 582306) (black circles) induces the chemotaxis of mouse Baf3-hCCR6 transfectant chemoattractant cells. Ultra-LEAF™ purified anti- mouse CCL20 (MIP-3α) antibody (clone A16054H) (purple squares) neutralizes the chemotaxis of recombinant mouse CCL20 (MIP-3α) at 0.3 ng/mL on mouse Baf3-hCCR6 cells in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.08 - 0.4 µg/mL.
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942903 100 µg £202.00
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942904 1 mg £650.00
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Description

CCL20 (MIP-3α) belongs to the CC chemokine family. Human CCL20 is a protein of 96 amino acids, which shares the highest homology of 28% amino acid identity with MIP-1β and 64% amino acid sequence identity with the mouse CCL20. CCL20 is produced by activated monocytes, T cells, neutrophils, endothelial cells, epithelial cells and fibroblasts. Its expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10. CCL20 is the ligand for chemokine receptor CCR6, which is expressed on memory T cells, B cells, and immature dendritic cells (DCs). Therefore, it is involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity. Upregulation of CCL20 plays important roles in autoimmune processes. This includes pathologies of the central nerve system (MS), pathogenesis of encephalomyelitis, psoriasis, rheumatoid arthritis, and inflammatory bowel diseases. CCL20 is also involved in various cancers, such as gastrointestinal cancers, pancreatic cancer, hepatocellular carcinoma, and breast cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CCL20 (MIP-3α)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CCL20 (MIP-3α) (Cat. No. 582306) at 0.3 ng/mL on mouse Baf3-hCCR6 transfectant chemoattractant cells. ND50 range: 0.08 - 0.4 µg /mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894527 (BioLegend Cat. No. 942903)
AB_2894527 (BioLegend Cat. No. 942904)

Antigen Details

Structure
Chemokine
Distribution

Monocytes, T cells, neutrophils, endothelial cells, fibroblasts, keratinocytes, synoviocytes, DCs, pulmonary and intestinal epithelial cells, dermal microvascular endothelial cells

Function
Chemoattracts immature dendritic cells at sites of injury, upregulated by mediators of inflammation such as TNF or LPS, induced by Th17 cytokines (IL-17A, IL-22, and TNF-α) in keratinocyte.
Interaction
T cells, Th17, Tregs, B cells, DC subsets including CD11b+ CD8α-myeloid DCs, Langerhans cells (LC), CD34+ cell-derived immature myeloid DCs, and immature monocyte-derived DCs
Ligand/Receptor
CCR6 (CD196)
Antigen References
  1. Varona R, et al. 1998. FEBS Lett. 440:188.
  2. Homey B, et al. 2000. J Immunol. 164:6621.
  3. Hoover DM, et al. 2002. J Biol Chem. 277:37647.
  4. Hirota K, et al. 2007. J Exp Med. 204:2803.
  5. Harper EG, et al. 2009. J Invest Dermatol. 129:2175.
  6. Ito T, et al. 2011. Exp Cell Res. 317:613.
Gene ID
20297 View all products for this Gene ID
UniProt
View information about CCL20 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/03/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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