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Ultra-LEAF™ Purified anti-human/mouse/rat MR1 Antibody

Pricing & Availability
Clone
26.5 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Complex Class I-related, HLALS
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
26.5_LEAF_MR1_Antibody_FC_012114
Human MR1-transfected Hela cell line was stained with LEAF™ purified MR1 (clone 26.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 26.5_LEAF_MR1_Antibody_FC_012114
    Human MR1-transfected Hela cell line was stained with LEAF™ purified MR1 (clone 26.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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361109 100 µg £125
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361110 1 mg £509
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Description

MHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Soluble complexes of MR1 ectodomain and B2M.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ELISA, IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines1-6, detection of folded MR1 conformers3,7, and immunoprecipitation of MR1 from cell lysates7.

Application References

(PubMed link indicates BioLegend citation)
  1. Young MH, et al. 2013. PLoS One. 8:e53789. (Block)
  2. Leeansyah E, et al. 2013. Blood 121:1124. (Block)
  3. Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA)
  4. Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC)
  5. Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC)
  6. Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC)
  7. Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)
Product Citations
  1. Sugimoto C, et al. 2022. Elife. 11:. PubMed
RRID
AB_2800999 (BioLegend Cat. No. 361109)
AB_2801000 (BioLegend Cat. No. 361110)

Antigen Details

Structure
Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
Distribution

MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.

Function
Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
Ligand/Receptor
α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14.
2. Young MH and Gapin L. 2013. Cell Res. 23:460.
3. Gold MC, et al. 2013. Mucosal Immunol. 6:35.
4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119.

Gene ID
3140 View all products for this Gene ID 15064 View all products for this Gene ID 25119 View all products for this Gene ID
UniProt
View information about MR1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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