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Ultra-LEAF™ Purified anti-human CD95 (Fas) Antibody

Pricing & Availability
Clone
DX2 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-64
Other Names
Fas, APO-1, TNFRSF6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DX2_LEAF_070507
Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified DX2, followed by anti-mouse IgGs FITC
  • DX2_LEAF_070507
    Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified DX2, followed by anti-mouse IgGs FITC
  • DX2_Purified_CD95_Antibody_IF_112012
    MCF7 breast cancer cells were stained with anti-human CD95 (clone DX2) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
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305655 100 µg £185
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305656 1 mg £509
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Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD95 transfected L cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICC - Verified
IHC, Apop - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
  3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
  4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
  5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
  6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
RRID
AB_2860812 (BioLegend Cat. No. 305655)
AB_2860813 (BioLegend Cat. No. 305656)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 45 kD
Distribution

T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes

Function
Mediates apoptosis
Ligand/Receptor
Fas ligand (CD178)
Cell Type
B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.

Gene ID
355 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD95 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD95 (Fas) DX2 FC
Biotin anti-human CD95 (Fas) DX2 FC
FITC anti-human CD95 (Fas) DX2 FC
PE anti-human CD95 (Fas) DX2 FC
PE/Cyanine5 anti-human CD95 (Fas) DX2 FC
Purified anti-human CD95 (Fas) DX2 FC,ICC,IHC
Alexa Fluor® 488 anti-human CD95 (Fas) DX2 FC
Alexa Fluor® 647 anti-human CD95 (Fas) DX2 FC
Brilliant Violet 421™ anti-human CD95 (Fas) DX2 FC
Pacific Blue™ anti-human CD95 (Fas) DX2 FC
PE/Cyanine7 anti-human CD95 (Fas) DX2 FC
Brilliant Violet 605™ anti-human CD95 (Fas) DX2 FC
PerCP/Cyanine5.5 anti-human CD95 (Fas) DX2 FC
Purified anti-human CD95 (Fas) (Maxpar® Ready) DX2 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD95 (Fas) DX2 FC
APC/Fire™ 750 anti-human CD95 (Fas) DX2 FC
APC/Cyanine7 anti-human CD95 (Fas) DX2 FC
Brilliant Violet 510™ anti-human CD95 (Fas) DX2 FC
Brilliant Violet 711™ anti-human CD95 (Fas) DX2 FC
Brilliant Violet 785™ anti-human CD95 (Fas) DX2 FC
Brilliant Violet 650™ anti-human CD95 (Fas) DX2 FC
Alexa Fluor® 700 anti-human CD95 (Fas) DX2 FC
TotalSeq™-A0156 anti-human CD95 (Fas) DX2 PG
TotalSeq™-C0156 anti-human CD95 (Fas) DX2 PG
TotalSeq™-B0156 anti-human CD95 (Fas) DX2 PG
Ultra-LEAF™ Purified anti-human CD95 (Fas) DX2 FC,ICC,IHC,Apop
TotalSeq™-D0156 anti-human CD95 (Fas) DX2 PG
PE/Fire™ 640 anti-human CD95 (Fas) DX2 FC
KIRAVIA Blue 520™ anti-human CD95 (Fas) DX2 FC
APC/Fire™ 810 anti-human CD95 (Fas) Antibody DX2 FC
PE anti-human CD95 DX2 FC
Spark YG™ 593 anti-human CD95 (Fas) DX2 FC
PE/Fire™ 700 anti-human CD95 (Fas) DX2 FC
PerCP/Fire™ 780 anti-human CD95 (Fas) DX2 FC
Spark Blue™ 574 anti-human CD95 (Fas) DX2 FC
Go To Top Version: 2    Revision Date: 01/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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