Ultra-LEAF™ Purified anti-human CD95 (FAS) Antibody

Pricing & Availability
Clone
A16086F (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor receptor superfamily member 6 (TNFRSF6), ALPS1A, APO-1, Apoptosis antigen 1 (APT1), CD95, FAS1, FASTM, FS-7-associated surface antigen
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16086F_ULEAF_CD95_Antibody_091917
Recombinant FASL induces cytotoxic effect in Jurkat human T cell leukemia cells in a dose dependent manner (grey circles). Anti-human - CD95 monoclonal antibody (clone A16086F, purple squares) blocks the cytotoxic effect induced by recombinant human FASL (60 ng/ml). The ND50 = 0.01 – 0.05 µg/ml.
  • A16086F_ULEAF_CD95_Antibody_091917
    Recombinant FASL induces cytotoxic effect in Jurkat human T cell leukemia cells in a dose dependent manner (grey circles). Anti-human - CD95 monoclonal antibody (clone A16086F, purple squares) blocks the cytotoxic effect induced by recombinant human FASL (60 ng/ml). The ND50 = 0.01 – 0.05 µg/ml.
Cat # Size Price Quantity Check Availability Save
684401 100 µg £185
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684402 1 mg £490
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Description

FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells.  FAS is a type I transmembrane protein and belongs to the TNF receptor superfamily. The extracellular region of the FAS possesses three cystein-rich domains characteristic of the TNF superfamily, and the intracellular region includes a death domain (DD). The binding of FASL to FAS induces conformational changes in FAS, leading to recruitment of FADD and procaspase-8, and assembly of the death-inducing signalling complex (DISC). Subsequently, caspase 8 is released from DISC and induces activation of caspase 3 and 7, proteolysis of cellular components, and apoptosis. Biological effects not based on apoptotic cell death have also been described. The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. Fas-activated DCs increase the expression of MHC II, B7 co-stimulatory molecules, and DC–lysosome-associated membrane protein (DC-LAMP), and secrete proinflammatory cytokines such as IL-1β and TNF-α. In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34+ cells and increases their colony formation. Human activated PBMC and tumor cell lines express full-length mRNA and several mRNA FAS variants derived by alternative splicing; deletion of an exon encoding the transmembrane domain results in a soluble FAS molecule that blocks apoptosis. Patients with systemic lupus erythematosus show high levels of soluble FAS.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human FAS
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Blocking - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking activity. The ND50 is 0.01– 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2716048 (BioLegend Cat. No. 684401)
AB_2716055 (BioLegend Cat. No. 684402)

Antigen Details

Structure
Trimer
Distribution

Activated human T cells,  B cells, malignant human lymphoid cell lines, fibroblasts, dendritic cells, thymocytes, macrophages, cardiomyocytes, hepatocytes, FAS is expressed on most cell types.

Function
Apoptotic clearance of virus-infected cells, contributes to shutdown of chronic immune responses and to maintenance of peripheral tolerance. Soluble FAS modulates cell apoptosis.
Interaction
Lymphocytes T (mainly CD8+), NK cells.
Ligand/Receptor
FASL (TNFSF6)
Cell Type
T cells, B cells, Fibroblasts, Dendritic cells, Thymocytes, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Oehm A, et al. 1992. J Biol Chem. 267:10709.
2. Cheng J, et al. 1994. Science. 263:1759. 
3. Papoff G, et al. 1996. J Immunol. 156:4622. 
4. Rescigno M, et al. 2000. J Exp Med. 192:1661. 
5. Giroux M, et al. 2005. Blood. 105:703. 
6. Kovacic N, et al. 2010. Expert Opin Ther Targets 14:1121.

Gene ID
355 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 09/20/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-human CD95 (FAS) Antibody

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