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Ultra-LEAF™ Purified anti-human CD289 (TLR9) Antibody

Pricing & Availability
Clone
S16013N (See other available formats)
Regulatory Status
RUO
Other Names
CD289, TLR9, Toll-like receptor 9
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
S16013N_ULEAF_CD289_TLR9_Antibody_1_062321.png
Human 293XL/TLR9 transfected cells were treated with CpG-B only (white bar) or Ultra-LEAF™ anti-human TLR9 (clone S16013N, purple bars), supernatant was harvested the next day and analyzed using the ELISA MAX™ Deluxe Set Human IL-8 (Cat. No. 431504).
  • S16013N_ULEAF_CD289_TLR9_Antibody_1_062321.png
    Human 293XL/TLR9 transfected cells were treated with CpG-B only (white bar) or Ultra-LEAF™ anti-human TLR9 (clone S16013N, purple bars), supernatant was harvested the next day and analyzed using the ELISA MAX™ Deluxe Set Human IL-8 (Cat. No. 431504).
  • S16013N_ULEAF_CD289_TLR9_Antibody_2_updated_062321.png
    Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right) followed by anti-rat IgG. Dot plots exclude monocytes.
  • S16013N_ULEAF_CD289_TLR9_Antibody_3_updated_062321.png
    Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right) followed by anti-rat IgG. Dot plots exclude monocytes.
  • S16013N_ULEAF_CD289_TLR9_Antibody_4_062321.png
    Human peripheral blood lymphocytes were stained with CD304 Brilliant Violet™ 421, CD19 APC and CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right), followed by anti-rat IgG. Dot plots exclude monocytes.
  • S16013N_ULEAF_CD289_TLR9_Antibody_5_062321.png
    Human peripheral blood lymphocytes were stained with CD304 Brilliant Violet™ 421, CD19 APC and CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right), followed by anti-rat IgG. Dot plots exclude monocytes.
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307703 100 µg £193
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Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen-associated molecular patterns (PAMPs). TLr9 recognizes unmethylated CpG sequences in DNA (i.e. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
FC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by blocking. > 80% blocking was observed with 5 µg antibody per well. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

S16013N clone can be used for blocking CpG-activated IL8 cytokine secretion (tested on 293XL/TLR9 cell line) and for intracellular and surface flow cytometry.

RRID
AB_2894671 (BioLegend Cat. No. 307703)

Antigen Details

Distribution

TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).

Function
TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References
  1. Hornung V, et al. 2002. J Immunol. 168:4531
  2. Eaton-Bassiri A, et al. 2004. Infect Immun. 72:7202.
  3. Krieg, A. 2007. Clin Invest. 117:1184.
  4. Decalf J, et al. 2007. J Exp Med. 2423:2437.
Gene ID
54106 View all products for this Gene ID
UniProt
View information about CD289 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/23/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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