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Purified Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG1-45 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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401401 50 µg £33
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401402 500 µg £89
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Description

The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ChIP - Verified
WB, IP, ICC, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozeki M, et al. 2008. J. Leukocyte Biol. 84:769. PubMed
  2. Kraetzel K, et al. 2008. Eur. Respair. J. 32:563. PubMed
  3. de Jong MA, et al. 2008. J Gen Virol. 89:2398. PubMed
  4. Romee R, et al. 2012 Blood. 120:4751. PubMed.
  5. Wong-Baeza I, et al. 2013. J. Immunol. 190:3216. PubMed.
Product Citations
  1. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  2. Frasca D, et al. 2020. Front Immunol. 11:392. PubMed
  3. Gabaev I, et al. 2020. Cell Rep. 33:108249. PubMed
  4. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  5. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  6. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  7. Wong-Baeza I, et al. 2013. J Immunol. 190:3216. PubMed
  8. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  9. Sithole N, et al. 2020. Retrovirology. 17:6. PubMed
  10. Evesson F, et al. 2010. J Biol Chem. 285:28529. PubMed
  11. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  12. Ai K, et al. 2022. PLoS Pathog. 18:e1010913. PubMed
  13. Wahlig S, et al. 2020. Sci Rep. 1.584027778. PubMed
  14. Sun Z, et al. 2017. J Physiol. 595:1987. PubMed
  15. Adeniran BV, et al. 2021. Hum Reprod. 36:1871. PubMed
  16. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  17. Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed
  18. Subramaniyan B, et al. 2021. Viruses. 13:. PubMed
  19. Pir GJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  20. Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed
  21. Han N, et al. 2021. STAR Protocols. 2(2):100460. PubMed
  22. Seoane M, et al. 2019. Oncogene. 38:3616. PubMed
  23. Ji Y, et al. 2012. J Biol Chem. 287:24378. PubMed
  24. Hangiu O, et al. 2022. iScience. 25:104958. PubMed
  25. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
  26. Essig F, et al. 2020. Int J Mol Sci. 21:00. PubMed
  27. Camilli G, et al. 2020. J Clin Invest. 130:4561. PubMed
  28. Huang CH, et al. 2019. ACS Nano. 13:11698. PubMed
  29. Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
  30. Munoz O, et al. 2022. PLoS Pathog. 18:e1010673. PubMed
  31. Barzilai S, et al. 2016. J Leukoc Biol. 99: 1045 - 1055. PubMed
  32. Vermi W, et al. 2018. Cancer Res. 78:3544. PubMed
  33. Satchwell TJ, et al. 2019. Nat Commun. 10:3806. PubMed
  34. Fetz AE, et al. 2021. Acta Biomater. 130:281. PubMed
  35. Lavie L, et al. 2022. Front Neurol. 13:811916. PubMed
  36. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  37. Nasser H, et al. 2022. STAR Protoc. 3:101773. PubMed
  38. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  39. Meyrath M, et al. 2020. Nat Commun. 2.564583333. PubMed
  40. Jong M, et al. 2008. J Gen Virol. 89:2398. PubMed
  41. Suzuki R, et al. 2022. Nature. . PubMed
  42. Rolfes V, et al. 2020. Cell Reports. 31(6):107615. PubMed
  43. Staessens S, et al. 2019. Haematologica. 104(10). PubMed
  44. Hori Y, et al. 2022. Front Cardiovasc Med. 9:981088. PubMed
  45. Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
  46. Doni A, et al. 2021. Nat Commun. 12:3739. PubMed
  47. Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed
  48. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  49. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  50. Demetriou P, et al. 2020. Nat Immunol. 1232:21. PubMed
  51. Camilli G, et al. 2018. Eur J Immunol. 48:757. PubMed
  52. Enomoto Y, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  53. Kraetzel K, et al. 2008. Eur Respir J. 32:563. PubMed
  54. Shively M 2008. J Leukoc Biol. 84:769. PubMed
  55. Parashar D, et al. 2019. Cell Rep. 29:4389. PubMed
  56. Uttagomol J, et al. 2019. Int J Mol Sci. 5.153472222. PubMed
  57. Yakymiv Y, et al. 2021. Sci Rep. 11:21230. PubMed
  58. Huang Y, et al. 2021. Front Immunol. 12:649502. PubMed
  59. Romee R, et al. 2012. Blood. 120:4751. PubMed
  60. Wülfing C, et al. 2018. Immun Inflamm Dis. 0.495833333. PubMed
  61. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  62. Kauer J, et al. 2019. Sci Rep. 9:18337. PubMed
  63. Espinosa-Díez C, et al. 2018. Redox Biol. 14:88. PubMed
RRID
AB_2801452 (BioLegend Cat. No. 401401)
AB_2801451 (BioLegend Cat. No. 401402)

Antigen Details

Gene ID
NA

Other Formats

View All Reagents
Description Clone Applications
Purified Mouse IgG1, κ Isotype Ctrl MG1-45 FC,ICFC,ICC,IHC,IP,WB,ChIP
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl MG1-45 FC,ICFC,WB,IP,ICC,IHC,FA
Go To Top Version: 3    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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