- A16075D (See other available formats)
- Regulatory Status
- Other Names
- Transmembrane protein 119
- Mouse IgG2b, κ
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- Product Citations
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Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.Product Details
- Antibody Type
- Host Species
- Recombinant human TMEM119 protein (extracellular domain)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
WB, ICC, IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.6 - 5.0 µg/mL. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron.
*The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.
(PubMed link indicates BioLegend citation)
Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed
- Product Citations
AB_2734646 (BioLegend Cat. No. 853301)
AB_2734647 (BioLegend Cat. No. 853302)
- TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues.
Cellular distribution: Membrane and endoplasmic reticulum.
- TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
- TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Antigen References
- Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113
- Satoh J, et al. 2016. Neuropathology. 36:39-49
- Kawao N and Kaji H. 2015. J Cell Biochem. 116:687
- Zrzavy T, et al. 2017. Brain. 140:1900-1913
- Gene ID
- 338773 View all products for this Gene ID
- View information about TMEM119 on UniProt.org
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