- B122 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
- Armenian Hamster IgG
- Ave. Rating
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- Product Citations
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IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- Armenian Hamster
- E. coli -expressed, recombinant mouse IL-1β
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8.
(PubMed link indicates BioLegend citation)
- Hogquist K, et al. 1991. J. Immunol. 146:1534.
- Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
- Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
- Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
- Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
- Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
- Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
- Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed
- Product Citations
AB_315286 (BioLegend Cat. No. 503501)
AB_315287 (BioLegend Cat. No. 503502)
- Cytokine; 17.5 kD (Mammalian)
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
- Cell Sources
- Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
- Cell Targets
- B cells, T cells, monocytes
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 16176 View all products for this Gene ID
- View information about IL-1beta on UniProt.org
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