Purified anti-mouse/rat CD42d Antibody

Pricing & Availability
Clone
1C2 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
1C2_Purified_CD42d_Antibody_FC_072814.jpg
C57BL/6 platelets were stained with purified 1C2 (clone 1C2, filled histogram) or Armenian hamster IgG isotype control (bottom), followed by biotinylated anti-Armenian hamster IgG biotin and Sav - PE.
  • 1C2_Purified_CD42d_Antibody_FC_072814.jpg
    C57BL/6 platelets were stained with purified 1C2 (clone 1C2, filled histogram) or Armenian hamster IgG isotype control (bottom), followed by biotinylated anti-Armenian hamster IgG biotin and Sav - PE.
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148501 50 µg £80
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Description

CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
  2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
  3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
RRID
AB_2564114 (BioLegend Cat. No. 148501)

Antigen Details

Structure
83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).
Distribution

Resting platelets and megakaryocytes.

Function
CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.
Ligand/Receptor
CD42d.
Cell Type
Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sivaraman B, et al. 2011. Biomaterials. 32:5365.
2. Berger G, et al. 1996. Blood. 87:1385.
3. Ravanat C, et al. 1997. Blood. 89:3253.
4. Andrews RK, et al. 1998. Biochemistry. 37:638.
5. Inoue O, et al. 2008. 283:16279.
6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327.
7. Shimomura T, et al. 1990. Blood. 75:2349.
8. Wu G, et al. 1997. Blood. 90:2660.

Gene ID
14729 View all products for this Gene ID
UniProt
View information about CD42d on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/12/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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