- 8.1.1 (See other available formats)
- Regulatory Status
- Other Names
- T1a, gp38, stromal cell marker
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
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The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Syrian Hamster
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F - Verified
SB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
(PubMed link indicates BioLegend citation)
- Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
- Farr AG, et al. 1992. J. Exp. Med. 176:1477.
- Bekiaris V, et al. 2008. J. Immunol. 180:6768.
- Algars A, et al. 2011. Blood 117:4387. PubMed
- Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
- Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
- Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_1089186 (BioLegend Cat. No. 127401)
AB_1089187 (BioLegend Cat. No. 127402)
- 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Expressed on epithelial and mesothelial cells.
- Cell Type
- Epithelial cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
- Gene ID
- 14726 View all products for this Gene ID
- View information about Podoplanin on UniProt.org
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
|Biotin anti-mouse Podoplanin||8.1.1||FC|
|Purified anti-mouse Podoplanin||8.1.1||FC, IHC-F, SB|
|Alexa Fluor® 488 anti-mouse Podoplanin||8.1.1||FC|
|PE anti-mouse Podoplanin||8.1.1||FC|
|APC anti-mouse Podoplanin||8.1.1||FC|
|PE/Cyanine7 anti-mouse Podoplanin||8.1.1||FC|
|Alexa Fluor® 594 anti-mouse Podoplanin||8.1.1||ICC, IHC-F|
|FITC anti-mouse Podoplanin||8.1.1||FC|
|APC/Cyanine7 anti-mouse Podoplanin||8.1.1||FC|
|PE/Dazzle™ 594 anti-mouse Podoplanin||8.1.1||FC|
|PerCP/Cyanine5.5 anti-mouse Podoplanin||8.1.1||FC|
|Brilliant Violet 421™ anti-mouse Podoplanin||8.1.1||FC|
|TotalSeq™-A1035 anti-mouse Podoplanin||8.1.1||PG|
|APC/Fire™ 750 anti-mouse Podoplanin||8.1.1||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse Podoplanin
Purified anti-mouse Podoplanin
Alexa Fluor® 488 anti-mouse Podoplanin
PE anti-mouse Podoplanin
APC anti-mouse Podoplanin
PE/Cyanine7 anti-mouse Podoplanin
Alexa Fluor® 594 anti-mouse Podoplanin
FITC anti-mouse Podoplanin
APC/Cyanine7 anti-mouse Podoplanin
PE/Dazzle™ 594 anti-mouse Podoplanin
PerCP/Cyanine5.5 anti-mouse Podoplanin
Brilliant Violet 421™ anti-mouse Podoplanin
TotalSeq™-A1035 anti-mouse Podoplanin
APC/Fire™ 750 anti-mouse Podoplanin