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Purified anti-mouse IL-6 Antibody

Pricing & Availability
Clone
MP5-20F3 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
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Product Citations
publications
MouseIL-6_061604
  • MouseIL-6_061604
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504501 50 µg £57
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504502 500 µg £95
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7-expressed, recombinant mouse IL-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, Neut, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.5 – 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1-3,11 or ELISPOT Capture4,5: The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.

Neutralization1-2,8-10: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506).

Additional reported applications (for relevant formats of this clone) include: In Vivo Capture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).

Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA Capture, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA Capture)
  4. Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181. (ELISPOT Capture)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT Capture)
  6. Litton M, et al. 1994. J. Immunol Meth. 175:47. (IHC)
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. (In Vivo Capture)
  8. Starnes HF Jr, et al. 1990. J. Immunol. 145:4185. (Neut)
  9. Riedemann NC, et al. 2003. J. Immunol. 170:503. (Neut)
  10. Rochman I, et al. 2005. J. Immunol. 174:4761. (Neut)
  11. O'Connell PJ, et al. 2006. Blood 107:1010. (ELISA Capture)
  12. Ozeki Y, et al. 2010. Int. Immunol. 22:179. PubMed
  13. Galie M, et al. 2005. Carcinogenesis 26:1868 (IHC)
  14. Schone MP, et al. 2000. J Immunol 165:6583 (IHC)
  15. Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed
  16. Notley CA, et al. 2015. J Immunol. 194:1621. PubMed
Product Citations
  1. Wu G, et al. 2020. Front Oncol. 10:194. PubMed
  2. Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
  3. Xu M et al. 2018. Immunity. 48(4):787-798 . PubMed
  4. Lee J, et al. 2015. PLoS One. 10: 0142624. PubMed
  5. Al Sayed MF, et al. 2019. Cancer Res. 79:346. PubMed
  6. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  7. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
  8. Morita M, et al. 2021. Heliyon. 7:e06228. PubMed
  9. Muraleedharan A, et al. 2021. Glia. 69:697. PubMed
  10. Peterson LW, et al. 2017. J Exp Med. 214:3171. PubMed
  11. Herrada AA, et al. 2022. Front Immunol. 13:841641. PubMed
  12. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  13. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  14. Di Conza G, et al. 2021. Nat Immunol. 22:1403. PubMed
  15. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
  16. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  17. Ji Z, et al. 2019. Front Immunol. 1.722916667. PubMed
  18. Ozeki Y, et al. 2010. Int Immunol. 1.040972222. PubMed
  19. Rosen DA, et al. 2019. Sci Transl Med. 11:eaau5266. PubMed
  20. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
RRID
AB_315335 (BioLegend Cat. No. 504501)
AB_315336 (BioLegend Cat. No. 504502)

Antigen Details

Structure
Neuropoietic cytokine family; 22-29 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-6 MP5-20F3 ICFC
Purified anti-mouse IL-6 MP5-20F3 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,IHC-F
APC anti-mouse IL-6 MP5-20F3 ICFC
Purified anti-mouse IL-6 (Maxpar® Ready) MP5-20F3 ELISA Capture,CyTOF®
Ultra-LEAF™ Purified anti-mouse IL-6 MP5-20F3 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,IHC-F
Go To Top Version: 2    Revision Date: 05/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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