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Purified anti-MAP2 Antibody (Previously Covance catalog# PCK-554P)

Pricing & Availability
Clone
Poly28225 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein 2, MAP-2
Previously
Covance Catalog# PCK-554P
Isotype
Chicken IgY
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Product Citations
publications
Poly28225_Purified_MAP2_Antibody_IHC_032615
Immunohistochemical staining of MAP2 Chicken Polyclonal Antibody, on formalin fixed paraffin embedded mouse brain tissue at 1/10,000 dilution.
  • Poly28225_Purified_MAP2_Antibody_IHC_032615
    Immunohistochemical staining of MAP2 Chicken Polyclonal Antibody, on formalin fixed paraffin embedded mouse brain tissue at 1/10,000 dilution.
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822501 50 µL £257
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Description

Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Human, Rat, Cow
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This antibody was made against MAP2 in chickens.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100,000*
• IF: 1:10,000

Application Notes

This antibody is effective in immunoblotting (WB) and immunofluorescence (IF). This antibody does not bind to protein A or protein G.

*Predicted MW = 280 kD and a doublet ~70 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Savas JN, et al. 2010. J Biol Chem. 285:13142-53. [IF] PubMed
Product Citations
  1. Bartoll A, et al. 2020. EMBO Mol Med. 12:e11776. PubMed
  2. Kodani Y, et al. 2022. Front Endocrinol (Lausanne). 13:941166. PubMed
  3. Lin YT et al. 2018. Neuron. 98(6):1141-1154 . PubMed
  4. Bresciani A, et al. 2018. PLoS One. 9:306. PubMed
  5. van Ineveld RL, et al. 2022. Nat Protoc. 17:3028. PubMed
  6. Tang QY, et al. 2020. Stem Cell Reports. 0.929861111. PubMed
  7. Ka M, Moffat J, Kim W 2016. Cereb Cortex. 10.1093/cercor/bhw319. PubMed
  8. Raja WK, et al. 2022. PLoS One. 17:e0277532. PubMed
  9. Savas J, et al. 2010. J Biol Chem. 285:13142-13153. PubMed
  10. Cui H, et al. 2022. STAR Protoc. 3:101813. PubMed
  11. Sepehrimanesh M, et al. 2021. STAR Protoc. 2:100917. PubMed
  12. Fischer M, et al. 2021. Alcohol Clin Exp Res. 45:979. PubMed
  13. Merlini M et al. 2019. Neuron. 101(6):1099-1108 . PubMed
  14. Gomez-Sintes R, et al. 2022. Nat Commun. 13:4220. PubMed
  15. Castagnola S, et al. 2020. Genome Res. 1633:30. PubMed
  16. Almansa D, et al. 2022. Int J Mol Sci. 23:. PubMed
  17. Sathler MF, et al. 2021. J Cell Sci. 134:. PubMed
RRID
AB_2564858 (BioLegend Cat. No. 822501)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Gene ID
4133 View all products for this Gene ID
UniProt
View information about MAP2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 11/01/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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