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Purified anti-human CD365 (Tim-1) Antibody

Pricing & Availability
Clone
1D12 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T cell immunoglobulin mucin 1, TIMD-1, KIM-1 (kidney injury molecule-1), HAVCR-1, CD365
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1D12_Pure_011712
769-P, human kidney cell line was stained with purified anti-human CD365 (Tim-1, clone 1D12) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 1D12_Pure_011712
    769-P, human kidney cell line was stained with purified anti-human CD365 (Tim-1, clone 1D12) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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353902 100 µg £157
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Description

CD365 (Tim-1) is a transmembrane glycoprotein expressed on activated CD4+ lymphocytes especially on Th2 cells. It has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is a hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in the costimulation of T cell proliferation. Tim-1 has been reported to be an endogenous ligand for LMIR5 (Leukocyte mono-immunoglobulin (Ig)-like receptor 5), and the Tim-1-LMIR5 interaction plays a physiological role in immune regulation by myeloid cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TIM-1-IgV Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) include: blocking function1.

Application References

(PubMed link indicates BioLegend citation)
  1. Freeman GJ, et al. 2007. Immunity. 27:927. (Block)
RRID
AB_11124741 (BioLegend Cat. No. 353902)

Antigen Details

Structure
Type 1 transmembrane glycoprotein.
Distribution

Activated CD4+ lymphocytes, especially on Th2 cells.

Function
TIM-1 ligation induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Ligand/Receptor
TIM-4
Cell Type
Dendritic cells, T cells, Th2
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Freeman GJ, et al. 2007. Immunity. 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mariat C, et al. 2005. Philos. Trans. R. Soc. Lond. B. 360:1681.
5. Yamanishi Y, et al. 2010. J. Exp. Med. 207:1501.

Gene ID
26762 View all products for this Gene ID
UniProt
View information about CD365 on UniProt.org
Go To Top Version: 2    Revision Date: 01/14/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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