Purified anti-human Adiponectin (Acrp30) Antibody

Pricing & Availability
Clone
62-9 (See other available formats)
Regulatory Status
RUO
Other Names
GBP-28, apM1, AdipoQ, and Acrp30
Isotype
Mouse IgG2a, κ
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Product Citations
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Cat # Size Price Quantity Check Availability Save
528701 50 µg £86.00
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528702 500 µg £222.00
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Description

Adiponectin, also referred as Acrp30, AdipoQ, apM1, and gelatin binding protein of 28 kD (GBP-28), is encoded by the ADIPOQ gene in humans. Adiponectin contains 244 amino acids with a collagen-like domain at the N-terminal and a globular domain at the C-terminal. The globular domain can be proteolytically truncated forming the globular adiponectin (gAdiponectin). This globular domain resembles the structure of the complement C1q and the TNF-α superfamily. Adiponectin exists in a wide range of homo-oligomers: a low-molecular weight (LMW) trimer, a medium molecular weight (MMW) hexamer, and a high molecular weight (HMW) multimer with little or no interconversion between the forms. Females generally have higher HMW than males in proportion and absolute amounts. Adiponectin has two receptors (adipoRs)-1 and -2. AdipoR1 mRNA is detectable in skeletal muscle, spleen, lung, heart, kidney, and liver. AdipoR2 is expressed in liver, but is also detectable in heart, lung, skeletal muscle, and kidney.

Adiponectin plays an important role in carbohydrate and lipid metabolism. It promotes insulin-sensitivity and protects cells from inflammation and apoptosis. It is also hypothesized to have anti-atherogenic activity. Normal circulating adiponectin level ranges from 4-30 µg/mL. Adiponectin levels are inversely correlated with body mass index (BMI). Low serum level of adiponectin is found in obesity, type 2 diabetes and those with coronary artery disease. Therefore, adiponectin serves as an important clinical biomarker for metabolic syndromes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Adiponectin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000 to 156 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified 62-9 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 112-1 antibody as the detection antibody and human Adiponectin protein as the standard.

For testing human Adiponectin (Acrp30) in serum, plasma, or supernatant, BioLegend’s ELISA LEGEND MAX™ Kits are specially developed and recommended.

RRID
AB_2571886 (BioLegend Cat. No. 528701)
AB_2571887 (BioLegend Cat. No. 528702)

Antigen Details

Structure
Protein hormone.
Distribution
Adipose tissue.
Function
Modulates a number of metabolic processes including glucose regulation and fatty acid oxidation.
Interaction
Liver, heart, lung, skeletal muscle, and kidney.
Ligand/Receptor
Adiponectin receptor 2
Biology Area
Complement
Molecular Family
Enzymes and Regulators
Antigen References

1. Ouchi N, et al. 2000. Circulation 102:1296.
2. Ouchi N, et al. 1999. Circulation 100:2473-6.
3. Nakano Y, et al. 1996. J. Biochem. 120:803.
4. Yokota T, et al. 2000. Blood 96:1723.

Gene ID
9370 View all products for this Gene ID
UniProt
View information about Adiponectin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/18/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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