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Purified anti-GIT1 Antibody

Pricing & Availability
Clone
W18253B (See other available formats)
Regulatory Status
RUO
Other Names
G Protein-Coupled Receptor Kinase Interacting, ArfGAP 1, GRK-Interacting Protein 1, ARF GAP GIT1, CAT1, Cool-associated and tyrosine phosphorylated protein 1, ARF GTPase-activating protein GIT1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18253B_PURE_GIT1_Antibody_1_022120
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GIT1 antibody (clone W18253B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending GIT1 expression; predicted expression data was obtained from Human Protein Atlas.
  • W18253B_PURE_GIT1_Antibody_1_022120
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GIT1 antibody (clone W18253B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending GIT1 expression; predicted expression data was obtained from Human Protein Atlas.
  • W18253B_PURE_GIT1_Antibody_2_021420
    A549 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) of either purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) (panel A) or purified anti-GIT1 antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • W18253B_PURE_GIT1_Antibody_3_022120
    Whole cell extracts (250 μg total protein) prepared from A549 cells were immunoprecipitated overnight with 2 μg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-GIT1 antibody (clone W18253B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1 µg/mL (1:500 dilution) of W18253B. Lane M: Molecular weight marker.
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935601 25 µg £70
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935602 100 µg £174
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Description

GIT1 is a G protein-coupled receptor kinase-interacting protein which is a component of the PIX/Rac/PAK pathway that is involved in cellular development. In this pathway, Rac activates PAK which leads to the phosphorylation of PIX and GIT1. The downstream result to these interactions leads to the formation of focal adhesion complexes in the periphery of the cell membrane with various proteins including FAK and paxillin. GIT1's various protein-protein interactions in the focal complex lead to downstream cellular events such as migration, morphogenesis and neuronal growth (spine/synapse formation).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human GIT1 protein of approximately 250 amino acids surrounding amino acid 500
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  • Non-specific bands present in HeLa lysates.
  • Triton X-100 is the best ICC permeabilization method for this antibody.
RRID
AB_2832898 (BioLegend Cat. No. 935601)
AB_2832899 (BioLegend Cat. No. 935602)

Antigen Details

Structure
GIT1 is a 761 amino acid protein with a predicted molecular weight of 84 kD.
Distribution

Cytosol and focal adhesion sites

Function
GTPase activation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Protein Trafficking and Clearance
Molecular Family
GTPases, Scaffold Proteins
Antigen References
  1. Zhang H, et al. 2005. J. Neurosci. 25:3379.
  2. Zhao ZS, et al. 2000. Mol. Cell. Biol. 20:6354.
  3. Goehler H, et al. 2004. Mol. Cell. 15:853.
Gene ID
28964 View all products for this Gene ID
UniProt
View information about GIT1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/13/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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