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Purified anti-GFP Epitope Tag Antibody (Previously Covance catalog# MMS-118P)

Pricing & Availability
Clone
B34 (See other available formats)
Regulatory Status
RUO
Other Names
Green Fluorescent Protein
Previously
Covance Catalog# MMS-118P
Isotype
Mouse IgG1
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Product Citations
publications
B34_Purified_GFP_Antibody_WB_032715
Western Blot showing specificity of Green Fluorescent Protein (GFP) (clone B34) 0.1 µg/ml . Lane 1: Molecular weight marker. Lane 2: 0.1 µg GFP. Lane 3: 0.05 µg GFP. Lane 4: BSA neg control.
  • B34_Purified_GFP_Antibody_WB_032715
    Western Blot showing specificity of Green Fluorescent Protein (GFP) (clone B34) 0.1 µg/ml . Lane 1: Molecular weight marker. Lane 2: 0.1 µg GFP. Lane 3: 0.05 µg GFP. Lane 4: BSA neg control.
Cat # Size Price Quantity Check Availability Save
902605 25 µL £61
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902601 200 µL £244
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902602 500 µL £381
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Description

Green Fluorescent Protein (GFP) is a single chain 238 amino acid protein derived from the jellyfish Aequoria victoria. GFP emits a bright green fluorescence when illuminated with UV or blue light and is a useful tool for cellular and developmental biologists. Unlike other bioluminescent molecules, GFP does not require additional proteins, substrates or co-factors for its activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Aequorea victoria, Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Antibody B34, generated against purified wild type GFP, offers an alternitive for detecting GFP and GFP variants including enhanced/mutant GFP.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, Purification
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:5,000*
 

Application Notes

This antibody is effective in immunoblotting (WB) of GFP labeled proteins.

*Predicted MW = 27 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  2. Kessels MM, et al. 2001. J Cell Biol. 153:351.
  3. Gerdes HH, Kaether C. 1996. FEBS Lett. 389:44.
  4. Davis I, et al. 1995. Dev Biol. 170:726.
  5. Chalfie M. 1995. Photochem Photobio. 62:651.
  6. Stearns T. 1995. Curr Biol. 5:262.
  7. Chalfie M, et al. 1994. Science. 263:802.
  8. Lavy M, et al. 2007. Curr. Biol. 17:947. (WB)
  9. Hobl B, et al. 2013. Protein Expr. Purif. 10:32. (WB)
  10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
  11. Asante D, et al. 2013. J. Cell. Sci. 126:5189. (WB) PubMed
  12. Oh Y, et al. 2013. Mol Biol Cell. 24:1305. (WB) PubMed
  13. Wloka C, et al. 2013. J. Cell. Biol. 200:271. (WB)
  14. Redfern RE, et al. 2010. Protein Sci. 19:1948. (WB) PubMed
Product Citations
  1. Bhatter N, et al. 2020. Wellcome Open Res. 3:102. PubMed
  2. Garnelo Gómez B, et al. 2021. J Cell Sci. 134:. PubMed
  3. Eshleman N, et al. 2016. RNA. 22: 1200 - 1214. PubMed
  4. Dhingani N, et al. 2020. Sci Rep. 10:18648. PubMed
  5. Costé de Bagneaux P, et al. 2020. PLoS Genet. 16:e1008625. PubMed
  6. Bauer CK, et al. 2019. Am J Hum Genet. 104:1139. PubMed
  7. Ulamec SM, et al. 2022. Nat Commun. 13:4986. PubMed
  8. Manjunath LE, et al. 2020. J Biol Chem. 295:17009. PubMed
  9. Shen K, et al. 2019. Mol Cell. 74:729. PubMed
  10. Shearer RF, et al. 2018. Mol Biol Cell. 29:1542. PubMed
  11. Stehle D, et al. 2018. Sci Rep. 8:12434. PubMed
  12. Bhatter N, et al. 2019. FEBS J. 286:4693. PubMed
  13. Arthur AL, et al. 2021. Elife. 10: . PubMed
  14. Herger A, et al. 2020. PLoS Genet. 16:e1008847. PubMed
  15. Kennedy SP, et al. 2019. Breast Cancer Res. 21:43. PubMed
  16. Sánchez-Solana B, et al. 2021. Mol Cancer. 20:141. PubMed
  17. Pogorelko G, et al. 2016. PLoS One. 11: 0159757. PubMed
  18. Sánchez-Martín J, et al. 2021. Nat Plants. 7:327. PubMed
  19. Croucher D, et al. 2016. Sci Signal. 9: ra69. PubMed
  20. Borgermann N et al. 2019. The EMBO journal. 38(8) pii: e101496. PubMed
  21. Kravchick D, et al. 2016. EMBO J. 35: 1923 - 1934. PubMed
  22. Redfern R, et al. 2010. Protein Sci. 19:1948-1956. PubMed
  23. Chattopadhyay S, et al. 2022. Nat Commun. 13:7184. PubMed
  24. Wang Y, et al. 2016. Sci Rep. 6:37213. PubMed
  25. Zhang Y, Barbieri Q 2007. J Biol Chem. 282:13022-13032. PubMed
  26. Schmitz F, et al. 2021. Sci Rep. 11:19232. PubMed
  27. Basic M, et al. 2021. J Biol Chem. 297:101263. PubMed
  28. Liu Y, et al. 2020. Nat Commun. 11:589. PubMed
  29. Sakamaki JI, et al. 2017. Mol Cell. 66:517. PubMed
  30. Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed
  31. Asante D, et al. 2013. J Cell Sci. 126:5189-5197. PubMed
  32. Oh Y, et al. 2013. Mol Biol Cell. 24:1305-1320. PubMed
  33. Zhang X, et al. 2016. Nat Commun. 7:12109. PubMed
  34. Manjunath LE, et al. 2020. J Biol Chem. 295:17009. PubMed
  35. den Brave F, et al. 2020. Cell Rep. 31:107680. PubMed
  36. McCaughey J, et al. 2019. J Cell Biol. 218:929. PubMed
RRID
AB_2734671 (BioLegend Cat. No. 902605)
AB_2565021 (BioLegend Cat. No. 902601)
AB_2565022 (BioLegend Cat. No. 902602)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about GFP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 09/20/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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