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Purified anti-FGF-4 Antibody

Pricing & Availability
Clone
2D1C57 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroslast Growth Factor 4 (FGF-4), Heparin-Binding Growth Factor 4 (HBGF-4), HST-1, K-FGF, KS3, Kaposi Sacrcoma Oncogene, Heparin Secretory Transforming Protein 1 (HSTF-1), FGF4, HBGF4, HST1, HSTF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2D1C57_PURE_FGF-4_Antibody_WB_063015
20 or 50 ng of human FGF-4 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-FGF-4 (clone 2D1C57) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 2D1C57_PURE_FGF-4_Antibody_WB_063015
    20 or 50 ng of human FGF-4 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-FGF-4 (clone 2D1C57) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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677402 100 µg £157
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Description

FGF-4 was first identified as HST-1 gene by a NIH3T3 transforming assay. It belongs to the FGF family that includes 22 members (FGF1-FGF23). However, FGF15 has not been identified in humans. FGF-4 exhibits strong effects on many different cell types and tissues, and plays an important role in both embryogenesis and adult tissue stem cell development in a variety of organisms. FGF-4 can stimulate limb mesenchyme proliferation and can provide all the signals required for normal outgrowth and patterning of the limb. FGF-4 mRNA has been detected in the apical ectodermal ridge of the limb bud in day 11 and 12 embryos. FGF-4 is also involved in early heart development by supporting the proliferation and differentiation of precardiac myoblasts. In adults, FGF-4 is expressed in the testis and its overexpression results in enhanced spermatogenesis. FGF-4 is also a potent inducer of platelet production from megakaryocytes. FGF-4 expression has been detected in the brain at both neonatal and adult stages. FGF-4 can induce neural stem cell proliferation and neuronal differentiation. The diverse functions of FGF-4 are mediated by FGF receptors (FGFR) that contain an extracellular ligand-binding domain with three immunoglobulin-like domains. Like other FGFs, FGF-4 also binds to anionic glycosaminoglycans heparin and heparin sulfate with a high affinity. Heparin sulfate availability has been shown to regulate the binding between FGF-4 and its receptors. The role of FGF-4 in cancer has also been extensively investigated. FGF-4 is an angiogenic protein, and the amplification of FGF-4 gene has been found in many human tumors. It has been shown that FGF-4 is a potential target for the treatment of human testicular tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human FGF-4 recombinant protein (54-206 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565907 (BioLegend Cat. No. 677402)

Antigen Details

Structure
The 153 amino acid recombinant protein has a predicted molecular mass of approximately 19.7 kD. Growth factor.
Distribution
Secreted. Expression of FGF-4 is highly restricted to undifferentiated stem cells.
Function
FGF-4 is a mitogenic, angiogenic, and survival factor. It is involved in cell proliferation and differentiation in embryonic development processes. N-glycosylation may negatively regulate FGF-4 activity.
Interaction
Precardiac myoblasts, megacariocytes, and neural stem cells.
Ligand/Receptor
FGFR-I, IIIc; FGFR-2, IIIc; FGFR-3, IIIc, and FGFR-4.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Growth Factors
Antigen References

1. Konishi H, et al. 1996. Oncogene 13:9.
2. Sun X, et al. 2000. Nat. Genet. 25:83.
3. Yamamoto H, et al. 2002. Oncogene 21:899.
4. Kosaka N, et al. 2006. FASEB J. 20:1484.
5. Kosaka N, et al. 2009. Dev. Dyn. 238:265.
6. Arao T, et al. 2013. Hepatology 57:1407.
7. Itoh N, Ohta H. 2013. Front Physiol. 4:247.

Gene ID
2249 View all products for this Gene ID
UniProt
View information about FGF-4 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/30/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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