Purified anti-Apo E Antibody (Previously Covance catalog# SIG-39740)

Pricing & Availability
Clone
D6E10 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Previously
Signet Catalog# 9740-02
Signet Catalog# 9740-05
Signet Catalog# 9740-10
Covance Catalog# SIG-39740
Isotype
Mouse IgG1
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Product Citations
publications
D6E10_PURE_Apo-E_Antibody_2_031220.png
IHC staining of purified anti-Apo E antibody (clone D6E10) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D6E10_PURE_Apo-E_Antibody_2_031220.png
    IHC staining of purified anti-Apo E antibody (clone D6E10) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D6E10_PURE_Apo-E_Antibody_3_031220.png
    IHC staining of purified anti-Apo E antibody (clone D6E10) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D6E10_PURE_Apo-E_Antibody_4_031220.png
    IHC staining of purified anti-Apo E antibody (clone D6E10) antibody on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat No. 405322) for one hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • D6E10_Purified_Apo-E_Antibody_080918
    Western blot of purified anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant protein; Lane 3: 40 ng of Apo E3 recombinant protein; Lane 4: 40 ng of Apo E4 recombinant protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Compare all formats
Cat # Size Price Quantity Check Availability Save
803301 200 µL £360
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803303 1 mL £1123
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

Application References

(PubMed link indicates BioLegend citation)
  1. Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108.
  3. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79.
  8. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
Product Citations
  1. McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
RRID
AB_2564662 (BioLegend Cat. No. 803301)
AB_2564661 (BioLegend Cat. No. 803303)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/12/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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