- 4B10 (See other available formats)
- Regulatory Status
- Other Names
- T-box expressed in T cells, T box 21, TBLYM
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
AB_1595593 (BioLegend Cat. No. 644805)
AB_1595488 (BioLegend Cat. No. 644806)
- T-box transcription factor, approximately 58 kD.
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- View information about T-bet on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-T-bet||4B10||WB, ICFC, IHC-F, IP|
|Alexa Fluor® 647 anti-T-bet||4B10||ICFC|
|Pacific Blue™ anti-T-bet||4B10||ICFC|
|Brilliant Violet 711™ anti-T-bet||4B10||ICFC|
|Brilliant Violet 421™ anti-T-bet||4B10||ICFC|
|Brilliant Violet 605™ anti-T-bet||4B10||ICFC|
|PE/Dazzle™ 594 anti-T-bet||4B10||ICFC|
|Purified anti-T-bet (Maxpar® Ready)||4B10||WB, CyTOF®|
|Alexa Fluor® 488 anti-T-bet||4B10||ICFC|
|Direct-Blot™ HRP anti-T-bet||4B10||WB|
|Brilliant Violet 785™ anti-T-bet||4B10||ICFC|
|Alexa Fluor® 594 anti-T-bet||4B10||ICC|
|KIRAVIA Blue 520™ anti-T-bet||4B10||ICFC|
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 647 anti-T-bet
Pacific Blue™ anti-T-bet
Brilliant Violet 711™ anti-T-bet
Brilliant Violet 421™ anti-T-bet
Brilliant Violet 605™ anti-T-bet
PE/Dazzle™ 594 anti-T-bet
Purified anti-T-bet (Maxpar® Ready)
Alexa Fluor® 488 anti-T-bet
Direct-Blot™ HRP anti-T-bet
Brilliant Violet 785™ anti-T-bet
Alexa Fluor® 594 anti-T-bet
KIRAVIA Blue 520™ anti-T-bet