PerCP/Cyanine5.5 anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
GK1-5_PerCPCyanine55_CD4_Antibody_082418
C57BL/6 mouse splenocytes were stained with CD3e FITC and CD4 (Clone GK1.5) PerCP/Cyanine5.5 (left), or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
  • GK1-5_PerCPCyanine55_CD4_Antibody_082418
    C57BL/6 mouse splenocytes were stained with CD3e FITC and CD4 (Clone GK1.5) PerCP/Cyanine5.5 (left), or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
100433 25 µg £68.00
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100434 100 µg £188.00
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, and immunoprecipitation1,2. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
Product Citations
  1. Tello‐Lafoz M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16370-w. PubMed
  2. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  3. Anderson AM, et al. 2018. J Immunol. 200:2057. PubMed
  4. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  5. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  6. Abhyankar MM, et al. 2018. NPJ Vaccines. 3:22. PubMed
  7. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  8. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  9. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  10. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  11. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  12. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  13. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  14. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  15. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  16. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  17. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  18. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  19. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  20. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  21. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  22. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  23. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  24. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  25. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  26. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  27. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  28. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  29. Wang F, et al. 2019. MAbs. 12:1685350. PubMed
  30. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  31. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  32. Grødeland G, et al. 2020. Front Immunol. 11:431. PubMed
  33. Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
  34. Wu HL, et al. 2018. J Immunol. 200:49. PubMed
  35. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  36. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  37. Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
  38. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  39. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  40. Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed
  41. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  42. Chen Z, et al. 2014. Cancer Immunol Res. 2:911. PubMed
  43. Pandey S, et al. 2014. J Immunol. 193:3632. PubMed
  44. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
  45. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  46. Flaherty S, Reynolds J 2015. J Vis Exp. 98: 52739. PubMed
  47. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  48. Kawano H, et al. 2016. Int Immunol. 28: 489 - 501. PubMed
  49. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  50. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  51. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  52. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  53. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  54. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  55. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
RRID
AB_893330 (BioLegend Cat. No. 100433)
AB_893324 (BioLegend Cat. No. 100434)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/24/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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