Loading...

PerCP/Cyanine5.5 anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
145-2C11_PerCPCyanine55_CD3e_Antibody_022219
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3e (clone 145-2C11) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • 145-2C11_PerCPCyanine55_CD3e_Antibody_022219
    C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3e (clone 145-2C11) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
100327 25 µg £57
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100328 100 µg £163
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Duarte J, et al. 2012. J Immunol. 189:1680. PubMed
  2. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  3. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  4. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  5. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  6. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  7. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  8. Feola S, et al. 2022. Front Immunol. 13:826164. PubMed
  9. Geng Z, et al. 2021. Nat Commun. 12:6584. PubMed
  10. Wu Q, et al. 2021. Cancer Commun (Lond). Online ahead of prin. PubMed
  11. Muthumani K, et al. 2015. Sci Transl Med. 7: 301ra132. PubMed
  12. Engler J, et al. 2017. Proc Natl Acad Sci U S A. 114(2):E181-E190. PubMed
  13. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  14. Girousse A et al. 2019. Cell Rep. 27(2):323-333 . PubMed
  15. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  16. Wu L, et al. 2022. Front Immunol. 13:860761. PubMed
  17. Helmersson S, et al. 2013. Am J Pathol. 182:1671. PubMed
  18. Di Luccia B, et al. 2020. Cell Host & Microbe. 27(6):899-908. PubMed
  19. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
  20. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  21. Das S, et al. 2019. Oncogene. 383316. PubMed
  22. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  23. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  24. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  25. Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed
  26. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  27. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  28. Katakam A, et al. 2015. Proc Natl Acad Sci U S A. 112: 14664 - 14669. PubMed
  29. Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
  30. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  31. Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed
  32. Sellau J, et al. 2016. Sci Rep. 6:28058. PubMed
  33. Härdle L, et al. 2015. Sci Rep. 5: 15112. PubMed
  34. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  35. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  36. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  37. Repáraz D, et al. 2022. J Immunother Cancer. 10:. PubMed
  38. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  39. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  40. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  41. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  42. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  43. Li H, et al. 2018. Nat Commun. 9:2830. PubMed
  44. Duan H, et al. 2021. J Clin Invest. 131:. PubMed
  45. Sibilio A, et al. 2022. iScience. 25:103790. PubMed
  46. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  47. Wang R, et al. 2022. Front Immunol. 13:947756. PubMed
  48. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  49. Muthalagu N, et al. 2020. Cancer Discov. 1.022222222. PubMed
  50. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  51. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  52. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  53. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  54. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  55. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  56. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  57. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  58. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  59. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  60. Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed
  61. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  62. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  63. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  64. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  65. Oh B, et al. 2017. Plast Reconstr Surg Glob Open. 5:e1595. PubMed
  66. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  67. Chen B, et al. 2021. Cell. 184:6262. PubMed
  68. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  69. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  70. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  71. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  72. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
  73. Young K, et al. 2021. Cell Stem Cell. . PubMed
  74. Gutierrez D, et al. 2014. Diabetes. 63:3827. PubMed
  75. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  76. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  77. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
RRID
AB_893320 (BioLegend Cat. No. 100327)
AB_893318 (BioLegend Cat. No. 100328)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 02/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account