- dG9 (See other available formats)
- Regulatory Status
- Other Names
- PRF1, P1, PFP, HPLH2
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Purified granules from the human lymphoma cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.
Does not cross-react with mouse1.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
- Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
- Watanabe N, et al. 1997. Blood 90:3662.
- Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
- Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
- Chen H, et al. 2005. J. Immunol. 175:591.
- Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
- Wood SM, et al. 2009. Blood 114:4117. PubMed
- Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
- Product Citations
AB_2169092 (BioLegend Cat. No. 308113)
AB_2284280 (BioLegend Cat. No. 308114)
- 70 kD
CTL, NK (cytoplasmic granules)
- Mediates targeted cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Antigen References
1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
- Gene ID
- 5551 View all products for this Gene ID
- View information about Perforin on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|FITC anti-human Perforin||dG9||ICFC|
|PE anti-human Perforin||dG9||ICFC|
|Purified anti-human Perforin||dG9||ICFC, IP, ICC, IHC|
|Alexa Fluor® 488 anti-human Perforin||dG9||ICFC|
|Alexa Fluor® 647 anti-human Perforin||dG9||ICFC|
|APC anti-human Perforin||dG9||ICFC|
|Pacific Blue™ anti-human Perforin||dG9||ICFC|
|PerCP/Cyanine5.5 anti-human Perforin||dG9||ICFC|
|Brilliant Violet 510™ anti-human Perforin||dG9||ICFC|
|Brilliant Violet 421™ anti-human Perforin||dG9||ICFC|
|Alexa Fluor® 594 anti-human Perforin||dG9||ICC|
|APC/Cyanine7 anti-human Perforin||dG9||ICFC|
|PE/Cyanine7 anti-human Perforin||dG9||ICFC|
|Brilliant Violet 711™ anti-human Perforin||dG9||ICFC|
|PE/Dazzle™ 594 anti-human Perforin||dG9||ICFC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human Perforin
PE anti-human Perforin
Purified anti-human Perforin
Alexa Fluor® 488 anti-human Perforin
Alexa Fluor® 647 anti-human Perforin
APC anti-human Perforin
Pacific Blue™ anti-human Perforin
PerCP/Cyanine5.5 anti-human Perforin
Brilliant Violet 510™ anti-human Perforin
Brilliant Violet 421™ anti-human Perforin
Alexa Fluor® 594 anti-human Perforin
APC/Cyanine7 anti-human Perforin
PE/Cyanine7 anti-human Perforin
Brilliant Violet 711™ anti-human Perforin
PE/Dazzle™ 594 anti-human Perforin