- TS2/16 (See other available formats)
- Regulatory Status
- V A-S202
- Other Names
- Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ß14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
- Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
- Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
- Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
- Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
- Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
- Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
- Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
- Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
- Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
- Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
AB_2715816 (BioLegend Cat. No. 303023)
AB_2715817 (BioLegend Cat. No. 303024)
- Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
- Cell-cell and cell-matrix interactions
- VCAM-1, MAdCAM-1, ECM
- Cell Type
- Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.
- Gene ID
- 3688 View all products for this Gene ID
- View information about CD29 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|APC anti-human CD29||TS2/16||FC|
|PE anti-human CD29||TS2/16||FC|
|PE/Cyanine5 anti-human CD29||TS2/16||FC|
|Purified anti-human CD29||TS2/16||FC, ICC, IHC, IP|
|APC/Cyanine7 anti-human CD29||TS2/16||FC|
|Alexa Fluor® 488 anti-human CD29||TS2/16||FC|
|Alexa Fluor® 647 anti-human CD29||TS2/16||FC|
|Alexa Fluor® 700 anti-human CD29||TS2/16||FC|
|Purified anti-human CD29 (Maxpar® Ready)||TS2/16||FC, CyTOF®|
|PerCP/Cyanine5.5 anti-human CD29||TS2/16||FC|
|PE/Cyanine7 anti-human CD29||TS2/16||FC|
|TotalSeq™-A0369 anti-human CD29||TS2/16||PG|
|TotalSeq™-C0369 anti-human CD29||TS2/16||PG|
|TotalSeq™-B0369 anti-human CD29||TS2/16||PG|
|PE/Dazzle™ 594 anti-human CD29||TS2/16||FC|
|Ultra-LEAF™ Purified anti-human CD29||TS2/16||FC, ICC, IHC, IP, Activ|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD29
PE anti-human CD29
PE/Cyanine5 anti-human CD29
Purified anti-human CD29
APC/Cyanine7 anti-human CD29
Alexa Fluor® 488 anti-human CD29
Alexa Fluor® 647 anti-human CD29
Alexa Fluor® 700 anti-human CD29
Purified anti-human CD29 (Maxpar® Ready)
PerCP/Cyanine5.5 anti-human CD29
PE/Cyanine7 anti-human CD29
TotalSeq™-A0369 anti-human CD29
TotalSeq™-C0369 anti-human CD29
TotalSeq™-B0369 anti-human CD29
PE/Dazzle™ 594 anti-human CD29
Ultra-LEAF™ Purified anti-human CD29