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PE Rat IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK4530 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
400607 25 µg £31
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400635 25 tests £39
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400608 100 µg £63
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400636 100 tests £68
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Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
test sizes: lot-specific; µg sizes: 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this rat IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <  0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
  2. Zeiser R, et al. 2007. Blood 109:2225.
  3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
  4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
  5. Yi H, et al. 2009. Blood 113:5819. PubMed
  6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
  7. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
  8. Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations
  1. Ojeda-Fernández L, et al. 2016. PLoS One. 12: 1005935. PubMed
  2. Nigam S, et al. 2020. Mol Imaging Biol. 22:685. PubMed
  3. Schuster C, et al. 2022. Front Immunol. 13:906499. PubMed
  4. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  5. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  6. Iwasaki Y, et al. 2016. PLoS One. 11: 0158282. PubMed
  7. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
  8. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  9. Madera L, et al. 2015. PLoS One. 10: 0133385. PubMed
  10. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  11. Catrina AM, et al. 2021. Rom J Morphol Embryol. 62:109. PubMed
  12. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
  13. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  14. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  15. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  16. Chen Y, et al. 2016. PLoS One. 11: 0149233. PubMed
  17. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  18. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  19. Klimovich B, et al. 2022. J Exp Clin Cancer Res. 41:80. PubMed
  20. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  21. Atcha H, et al. 2021. Front Immunol. 12:689397. PubMed
  22. Graham K, et al. 2011. Am J Pathol. 178:2716. PubMed
  23. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  24. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  25. Yang H, et al. 2022. Nutrients. 14:. PubMed
  26. Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
  27. Krishnamurthy B, et al. 2012. Diabetes. 61:425. PubMed
  28. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  29. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  30. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  31. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  32. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  33. Xu X, et al. 2018. J Dermatol Sci. 91:134. PubMed
  34. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  35. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  36. Takizawa S, et al. 2022. Mol Med. 28:91. PubMed
  37. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  38. Yang T, et al. 2022. Antioxidants (Basel). 11:. PubMed
  39. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  40. Izotova N, et al. 2021. Nat Commun. 12:1622. PubMed
  41. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  42. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  43. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  44. Zhang M, et al. 2019. Autophagy. 1.423611111. PubMed
  45. Hung G, et al. 2022. NPJ Aging. 8:10. PubMed
  46. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  47. Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
  48. Walter RJ, et al. 2022. Cell Death Dis. 13:168. PubMed
RRID
AB_326551 (BioLegend Cat. No. 400607)
AB_326552 (BioLegend Cat. No. 400608)
AB_893669 (BioLegend Cat. No. 400636)

Antigen Details

Gene ID
NA

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Biotin Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
FITC Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC,ICC,IHC,IP,WB
PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Pacific Blue™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PerCP Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl RTK4530 IHC-F
GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC,ICC,IHC,IP,WB,FA
APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Spark PLUS UV™ 395 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2b, κ Isotype Ctrl

  • Biotin Rat IgG2b, κ Isotype Ctrl

  • FITC Rat IgG2b, κ Isotype Ctrl

  • PE Rat IgG2b, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl

  • Purified Rat IgG2b, κ Isotype Ctrl

  • PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl

    RTK4530_Alx700_080408.jpg
    CD57BL/6 mouse splenocytes stained with RTK4530 Alexa Fluor®...

  • PerCP Rat IgG2b, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl

  • Spark PLUS UV™ 395 Rat IgG2b, κ Isotype Ctrl

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