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PE Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
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400507 25 µg £34
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400508 100 µg £68
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  3. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  4. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  5. Barkaway A, et al. 2021. Immunity. . PubMed
  6. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
  7. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
  8. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  9. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
  10. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  11. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  12. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  13. Madera L, et al. 2015. PLoS One. 10: 0133385. PubMed
  14. Zape J, et al. 2017. Cell Cycle. 10.1080/15384101.2017.1361569. PubMed
  15. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  16. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
  17. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  18. Hauptmann M, et al. 2017. Infect Immun . 10.1128/IAI.00156-17. PubMed
  19. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  20. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  21. Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
  22. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  23. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
  24. Toda S, et al. 2014. Blood. 123:3963. PubMed
  25. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  26. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
  27. Chung H, et al. 2021. Nat Methods. 18:1204. PubMed
  28. Takayanagi Y, et al. 2020. J Clin Med. 9:00. PubMed
  29. Kubiniok P, et al. 2019. Mol Cell Proteomics. 18:408. PubMed
  30. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  31. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  32. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  33. Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
  34. Sakurai T, et al. 2021. Life Sci Alliance. 4:. PubMed
  35. Yang H, et al. 2022. Nutrients. 14:. PubMed
  36. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  37. Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed
  38. Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed
  39. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  40. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  41. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  42. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  43. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  44. Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
  45. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  46. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  47. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  48. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  49. Rajashekara G, et al. 2008. J Bacteriol. 190:6243. PubMed
  50. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
  51. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  52. Yang Z, et al. 2020. J Invest Dermatol. 544:140. PubMed
  53. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  54. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  55. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  56. Grisanti LA, et al. 2019. JCI Insight. 5. PubMed
  57. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  58. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  59. Schmidt N, et al. 2011. J Immunol. 186:5873. PubMed
  60. Arya SB, et al. 2018. J Biol Chem. 293:9892. PubMed
  61. Sockolosky J, et al. 2016. Proc Natl Acad Sci U S A. 113: 2646 - 2654. PubMed
  62. Adamopoulos I, et al. 2015. Ann Rheum Dis. 74:1284. PubMed
  63. Li ZG, et al. 2018. Mil Med Res. 5:24. PubMed
  64. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  65. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  66. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  67. Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed
  68. Asson-Batres M, et al. 2016. Am J Physiol Heart Circ Physiol. 310: 1773 - 1789. PubMed
  69. Harada Y, et al. 2019. Pain. 160:2050. PubMed
  70. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  71. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
  72. Wu KKL, et al. 2021. Nat Commun. 12:6637. PubMed
  73. Nott A, et al. 2021. Nature Protocols. 16(3):1629-1646. PubMed
RRID
AB_326530 (BioLegend Cat. No. 400508)

Antigen Details

Gene ID
NA

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

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Description Clone Applications
APC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Biotin Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
FITC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA,ChIP
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Pacific Blue™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl RTK2758 ICC
GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl RTK2758 SB
PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2a, κ Isotype Ctrl

  • Biotin Rat IgG2a, κ Isotype Ctrl

  • FITC Rat IgG2a, κ Isotype Ctrl

  • PE Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl

  • Purified Rat IgG2a, κ Isotype Ctrl

    RTK2758_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl

  • PerCP Rat IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl

  • Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl

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