PE/Cyanine7 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_PECy7_090507
C57BL/6 mouse splenocytes stained with 30-F11 PE/Cyanine7
  • 30-F11_PECy7_090507
    C57BL/6 mouse splenocytes stained with 30-F11 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
103113 25 µg £55.00
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103114 100 µg £123.00
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Wang B, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.03.041. PubMed
  2. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  3. Chang T, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006485. PubMed
  4. Wang B,et al. 2017. Cell Discov.. 10.1038/celldisc.2017.36. PubMed
  5. Ishihara J, et al. 2018. Nat Commun. 9:2163. PubMed
  6. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  7. Garber C, et al. 2018. Nat Immunol. 19:151. PubMed
  8. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  9. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  10. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  11. Kaczmarek–Hájek K, et al. 2018. Elife. 7:e36217. PubMed
  12. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  13. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  14. Solanas G, et al. 2017. Cell. 170:678. PubMed
  15. Son NH, et al. 2018. J Clin Invest. 128:4329. PubMed
  16. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  17. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  18. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  19. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  20. Kellish P, et al. 2019. J Clin Invest. 130:2279. PubMed
  21. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  22. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  23. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  24. McGowan SE, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 315:L102. PubMed
  25. Kimbrough D, et al. 2018. J Mol Cell Cardiol. 119:51. PubMed
  26. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  27. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  28. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  29. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  30. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  31. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  32. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  33. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  34. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  35. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  36. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  37. Smith NR, et al. 2017. Cell Mol Gastroenterol Hepatol. 0.395138889. PubMed
  38. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  39. Gu H, et al. 2018. Aging (Albany NY). 1.525694444. PubMed
  40. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  41. Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
  42. Ghosh S, et al. 2019. Commun Biol. 0.325. PubMed
  43. Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed
  44. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  45. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  46. Wu JH, et al. 2020. EMBO Mol Med. 12:e10154. PubMed
  47. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  48. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  49. Shi Y, et al. 2020. Med Sci Monit. 26:e923328. PubMed
  50. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  51. Pan J, et al. 2020. J Neuroinflammation. 17:97. PubMed
  52. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  53. Galvão I, et al. 2018. Front Immunol. 9:142. PubMed
  54. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  55. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  56. Muri J, et al. 2020. Cell Reports. 30(13):4399-4417. PubMed
  57. Wu H, et al. 2020. Cell. 180(1):107-121.e17.. PubMed
  58. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  59. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  60. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  61. Yen Y, et al. 2008. J Virol. 82:12312. PubMed
  62. Mizuochi C, et al. 2012. PLoS One. 7:e35763. PubMed
  63. Ishii H, et al. 2012. Cell Death Dis. 9;3:e363. PubMed
  64. Gigliotti J, et al. 2013. J Am Soc Nephrol. 24:1451. PubMed
  65. Magness S, et al. 2013. Am J Physiol Gastrointest Liver Physiol. 305:542. PubMed
  66. Allen S, et al. 2011. J Virol. 85:11448. PubMed
  67. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  68. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  69. Jaworska K, et al. 2015. J Immunol. 194:325. PubMed
  70. Hussein M, et al. 2015. PLoS One. 10: 0135218. PubMed
  71. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  72. McCoy S 2015. Am J Physiol Lung Cell Mol Physiol. 309: L463-L474. PubMed
  73. Gigliotti J, et al. 2015. J Am Soc Nephrol. 26: 2470 - 2481. PubMed
  74. Hilliard K, et al. 2015. Infect Immun . 83: 4015 - 4027. PubMed
  75. Wu X, et al. 2016. Sci Rep. 6:18685. PubMed
  76. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  77. Khare A, et al. 2016. Cell Rep. 15: 1700-1714. PubMed
  78. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  79. Britschgi A, et al. 2017. Nature. 541:541-545. PubMed
  80. Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed
  81. Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed
  82. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  83. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  84. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  85. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  86. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  87. Gerner-Mauro KN, et al. 2020. Proc Natl Acad Sci U S A. 117:12182. PubMed
  88. Cain MP, et al. 2020. Dis Model Mech. 13:00. PubMed
  89. Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
  90. Jiang YH, et al. 2020. J Mol Cell Cardiol. 138:115. PubMed
  91. Sheng J, et al. 2021. eLife. 10:00. PubMed
  92. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  93. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  94. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  95. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  96. Kimmel JC, et al. 2021. Cell Reports. 35(4):109046. PubMed
  97. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  98. DellOrso S, et al. 2021. STAR Protocols. 2(2):100451. PubMed
RRID
AB_312978 (BioLegend Cat. No. 103113)
AB_312979 (BioLegend Cat. No. 103114)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/14/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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