PE anti-mouse H-2Kd Antibody

Pricing & Availability
Clone
SF1-1.1 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
SF1-1dot1_PE_020808
Balb/c mouse splenocytes stained with SF1-1.1 PE
  • SF1-1dot1_PE_020808
    Balb/c mouse splenocytes stained with SF1-1.1 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
116607 50 µg £68.00
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116608 200 µg £198.00
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Description

The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse d haplotype, Weakly cross-reacts with H-2k haplotype
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c Mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of Kd.

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2.

Application References

(PubMed link indicates BioLegend citation)
  1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
  2. Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
  3. Bashuda H, et al. 1997. Transplantation 63:113.
  4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
  6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
  8. Delon J, et al. 1998. Immunity 9:467.
Product Citations
  1. Du R, et al. 2018. Cell Death Dis. 9:126. PubMed
  2. Liu B, et al. 2019. Theranostics. 8:5801. PubMed
  3. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  4. Allen L 2004. J Immunol. 173:835. PubMed
RRID
AB_313742 (BioLegend Cat. No. 116607)
AB_313743 (BioLegend Cat. No. 116608)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
  3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.
Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kd on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/09/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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