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PE anti-human CD9 Antibody

Pricing & Availability
Clone
HI9a (See other available formats)
Regulatory Status
RUO
Workshop
V P018
Other Names
Tetraspanin, MRP-1, DRAP-24
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI9a_PE_060807
Human peripheral blood platelets stained with HI9a PE
  • HI9a_PE_060807
    Human peripheral blood platelets stained with HI9a PE
Compare all formats See PE spectral data
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312105 25 tests £84
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312106 100 tests £184
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Description

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rabbit, Rhesus, Sheep
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Valentijn A, et al. 2013. Hum Reprod. 28:2695. PubMed
  2. Han Y, et al. 2021. Exp Ther Med. 22:938. PubMed
  3. Liu Y, et al. 2022. Signal Transduct Target Ther. 7:347. PubMed
  4. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  5. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  6. Ricklefs FL, et al. 2020. Int J Mol Sci. 21:. PubMed
  7. Khan K, et al. 2022. J Tissue Eng Regen Med. 16:110. PubMed
  8. Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed
  9. Zabegina L, et al. 2021. Cells. 10:. PubMed
  10. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  11. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  12. Delgado-Peraza F, et al. 2021. Cells. 10:. PubMed
  13. Etzerodt A, et al. 2017. Sci Rep. 7:40286. PubMed
  14. Huygens C, et al. 2015. J Biol Chem. 290: 20105-20116. PubMed
  15. Smith-Díaz CC, et al. 2021. Front Oncol. 11:709543. PubMed
  16. Mastoridis S, et al. 2018. Front Immunol. 1.474305556. PubMed
RRID
AB_2075893 (BioLegend Cat. No. 312105)
AB_2075892 (BioLegend Cat. No. 312106)

Antigen Details

Structure
Tetraspan family, type III transmembrane protein, 24 kD
Distribution

Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells

Function
Adhesion, platelet activation, B cell development
Ligand/Receptor
Associates with CD63, CD81, CD82 and CD36, binds PSG17
Cell Type
B cells, Embryonic Stem Cells, Endothelial cells, Epithelial cells, Granulocytes, Lymphocytes, Platelets
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Miao WM, et al. 2001. Blood 97:1689.
2. Ellerman DA, et al. 2003. Mol Biol Cell. 14:5098.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

Gene ID
928 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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