PE anti-human CD197 (CCR7) Antibody

Pricing & Availability
Clone
G043H7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR2, CDw197, EBI1, CMKBR7
Isotype
Mouse IgG2a, κ
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Product Citations
publications
G043H7_PE_1_101111
Human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and CCR7/CD197 (clone G043H7) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
  • G043H7_PE_1_101111
    Human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and CCR7/CD197 (clone G043H7) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
  • G043H7_PE_2_101111
See PE spectral data View NHP data of the APC format.
Cat # Size Price Quantity Avail. Save
353203 25 tests £65.00
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353204 100 tests £161.00
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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  2. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  3. Cappellano G, et al. 2018. PLoS One. 13:e0192108. PubMed
  4. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  5. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  6. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  7. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  8. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  9. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  10. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  11. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  12. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  13. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  14. Christiansen M, et al. 2020. Front Immunol. 10:3022. PubMed
  15. Tavira B, et al. 2018. J Diabetes Res. 2018:9391845. PubMed
  16. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  17. Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
  18. Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
  19. Borowicz S, et al. 2021. PLOS ONE. 16(6):e0252197. PubMed
  20. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
RRID
AB_10916391 (BioLegend Cat. No. 353203)
AB_10913813 (BioLegend Cat. No. 353204)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 2    Revision Date: 04/08/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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