PE anti-human CD19 Antibody

Pricing & Availability
Clone
4G7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
B4, CVID3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4G7_PE_CD19_Antibody_071618
Human peripheral blood lymphocytes were stained with CD19 (clone 4G7) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).
  • 4G7_PE_CD19_Antibody_071618
    Human peripheral blood lymphocytes were stained with CD19 (clone 4G7) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
392505 25 tests £65
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392506 100 tests £136
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Chronic Lymphocytic Leukemia (CLL) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Cabezudo E, et al. 1999. Haematologica. 84:413-8
  2. Van Zelm MC et al. 2010. J Clin Invest. 120:1265-74
Product Citations
  1. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  2. Lu Y, et al. 2020. STAR Protoc. 1:100040. PubMed
  3. Li H, et al. 2020. Mol Ther Methods Clin Dev. 1.436111111. PubMed
RRID
AB_2750096 (BioLegend Cat. No. 392505)
AB_2750097 (BioLegend Cat. No. 392506)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

Normal and malignant B lymphocytes

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Lymphocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Tedder T, et al. 1994. Immunol. Today 15:437.
  2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/17/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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