Pacific Blue anti-mouse Lineage Cocktail with Isotype Ctrl

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Clone: 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status: RUO
Other Names: Lin-1
Isotype: Rat IgG2a/Rat IgG2b
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Product Citations: publications

Lineage_Cocktail_PB_1_112811 — C57BL/6 bone marrow cells were stained with CD117 FITC and Pacific Blue™ lineage cocktail (top plot) or Pacific Blue™ isotype control cocktail (bottom plot).

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133305

25 tests

£201

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133306

100 tests

£401

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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Pacific Blue™ Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. Pacific Blue™ anti-mCD3/ Pacific Blue™ anti-mLy-6G(Ly-6C)/ Pacific Blue™ anti-mCD11b/ Pacific Blue™ anti-mCD45R(B220)/ Pacific Blue™ anti-mTer-119
2. Pacific Blue™ Rat IgG2b/Rat IgG2a isotype controls

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Host Species: Rat
Formulation: This two-reagent set contains Pacific Blue™ conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation: The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling: The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_11126168 (BioLegend Cat. No. 133305)

Antigen Details

Distribution: T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes
Cell Type: B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Antigen References: 1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about Lineage on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Lineage Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Pacific Blue™ anti-mouse Lineage Cocktail	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Brilliant Violet 421™ anti-mouse Lineage Cocktail	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 07/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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