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ELISA MAX™ Standard Set Mouse IgE

Pricing & Availability
Regulatory Status
RUO
Other Names
mouse IgE ELISA kit
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Product Citations
publications
mouse IgE_122109
  • mouse IgE_122109
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432401 5 plates £182
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum, Plasma and cell culture supernatant Immunoglobulin E (IgE) Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum, plasma and cell cultures.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IgE ELISA MAX™ Capture Antibody (200X)
  • Mouse IgE ELISA MAX™ Detection Antibody (200X)
  • Mouse IgE Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Wang W, et al. 2007. J. Immunol. 178:4885.
Product Citations
  1. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  2. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  3. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  4. Van Dyken S, et al. 2016. Nat Immunol. 17:1381-1387. PubMed
  5. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  6. Koizumi SI, et al. 2018. Nat Commun. 9:5344. PubMed
  7. Sun Z, et al. 2020. Front Immunol. 1.568055556. PubMed
  8. Sukseree S, et al. 2021. J Allergy Clin Immunol. 147:2386. PubMed
  9. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  10. Zheng HC, et al. 2020. J Asthma Allergy. 0.895138889. PubMed
  11. Ishihara N, et al. 2022. Front Allergy. 3:996657. PubMed
  12. Spear S, et al. 2019. Front Immunol. 10:542. PubMed
  13. Lim JY, et al. 2020. Stem Cell Res Ther. 0.634722222. PubMed
  14. Xu C, et al. 2021. J Immunol. 206:2088. PubMed
  15. Tsuji T, et al. 2016. Allergol Int. 65: 172-179. PubMed
Standard Range
0.156-10 ng/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

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Go To Top Version: 2    Revision Date: 08/08/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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