LEGENDplex™ Human Cystatin C Capture Bead B9, 13X

Pricing & Availability
Regulatory Status
RUO
Other Names
CST3, Cystatin 3, Neuroendocrine Basic Polypeptide, Post-Gamma-Globulin, Gamma-Trace, Epididymis Secretory Protein Li 2, BA218C14.4, HEL-S-2, ARMD11
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Product Citations
publications
Cat # Size Price  
740566 100 tests (270 µL) £81
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To purchase, view our LEGENDplex™ Mix and Match page

Description

The Vascular Inflammation Panel 1 Mix and Match Capture Beads offer flexible customization within the panel (13-plex) with the following items listed below:

740555 LEGENDplex™ Human Myoglobin Capture Bead A4, 13X
740556 LEGENDplex™ Human MRP8/14 Capture Bead A5, 13X
740557 LEGENDplex™ Human NGAL Capture Bead A6, 13X
740558 LEGENDplex™ Human CRP Capture Bead A7, 13X
740559 LEGENDplex™ Human MMP-2 Capture Bead A8, 13X
740560 LEGENDplex™ Human OPN Capture Bead A10, 13X
740561 LEGENDplex™ Human MPO Capture Bead B2, 13X
740562 LEGENDplex™ Human SAA Capture Bead B3, 13X
740476 LEGENDplex™ Human IGFBP-4 Capture Bead B4, 13X
740563 LEGENDplex™ Human ICAM-1 Capture Bead B5, 13X
740564 LEGENDplex™ Human VCAM-1 Capture Bead B6, 13X
740565 LEGENDplex™ Human MMP-9 Capture Bead B7, 13X
740566 LEGENDplex™ Human Cystatin C Capture Bead B9, 13X

 

Selected items will be used together with:

740554 LEGENDplex™ Human Vascular Inflammation Panel 1 Standard
740553 LEGENDplex™ Human Vascular Inflammation Panel 1 Detection Antibodies
740374 LEGENDplex™ Buffer Set C
740377 or 740379 Filter Plate or V-bottom Plate

 

The assembled Human Vascular Inflammation Mix and Match Panel 1 is a multiplex bead-based assay kit, using fluorescence–encoded beads suitable for use on various flow cytometers. This allows simultaneous quantification of up to 13 human proteins: Myoglobin, MRP8/14, NGAL, CRP, MMP-2, OPN, MPO, SAA, IGFBP-4, ICAM-1, VCAM-1, MMP-9, and Cystatin C. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum, plasma, tissue culture supernatant, and urine samples.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.

Antigen Details

Biology Area
Cancer Biomarkers, Cell Biology
Molecular Family
Enzymes and Regulators
Gene ID
1471 View all products for this Gene ID
UniProt
View information about Cystatin C on UniProt.org

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 0    Revision Date: 06/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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