LEGEND MAX™ Human PD-L2 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Human PD-L2, Programmed cell death 1 ligand 2, CD273, PDCD1LG2, B7-DC
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447607 1 Pre-coated Plate £308.00
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Description

PD-L2 (CD273, PDCD1LG2, B7-DC) and its related protein PD-L1 are ligands for the Programmed Cell Death Protein 1 (PD1). A wide variety of immune and non-immune cells can express PD-L2, but it is mainly expressed in dendritic cells. Through its interaction with PD1, PD-L2 can act as an immune checkpoint molecule by negatively regulating T cell proliferation and cytokine production. However, many studies reported that PD-L2 can also stimulate T cell proliferation through interaction with receptors other than PD1. High level of PD-L2 expression can promote tumor metastasis because of its ability to suppress T cells-mediated anti-tumor activity. Moreover, increased PD-L2 expression might dampen the effectiveness of anti-PD-L1 treatments.

The LEGEND MAX™ Human PD-L2 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with anti-human PD-L2 monoclonal antibody. The detection antibody is a biotinylated anti-human PD-L2 monoclonal antibody. This kit is specifically designed for the accurate quantitation of human PD-L2 from serum and plasma. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human PD-L2 Pre-coated 96-well Strip Microplate
  • Human PD-L2 Detection Antibody
  • Human PD-L2 Standard
  • Avidin HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA - Quality tested

Sensitivity
0.35 ± 0.26 pg/mL
Standard Range
3.9 - 250 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paher or software data analysis
  • Polypropylene tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 03/11/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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