- Regulatory Status
- Other Names
- Migration inhibitory factor-related protein 14, S100 Calcium-Binding Protein A9, S100A9, Calgranulin B
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- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|445307||1 Pre-coated Plate||£291.00|
Migration inhibitory factor-related protein 14 (MRP14), also known as S100 Calcium-Binding Protein A9 (S100A9) and calgranulin B, is a 14 kD member of the S100 family, which is a group of calcium-binding proteins that regulate intra- and extracellular activities of other effector proteins, enzymes, receptors, cytoskeletal subunits, and nucleic acids. MRP14 consists of 114 amino acids and is expressed in granulocytes, monocytes, macrophages, and tumor cells. This protein predominantly forms heterodimers (known as calprotectin) and heterotetramers with MRP8 (S100A8), but also can form homodimers.
Human MRP14 shares significant amino acid sequence identity with MRP14 from other species, including >79%, 72%, 70%, 68%, 64%, and 57% from non-human primates, equine, bovine, porcine, rat, and mouse, respectively.
MRP14 has major roles as an inflammatory mediator. It regulates myeloid cell function by binding to Toll-like receptor-4 and the receptor for advanced glycation end-products (RAGE). MRP14 induces neutrophil chemotaxis and adhesion, promotes phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2, and induces degranulation of neutrophils by a MAPK-dependent mechanism. MRP14 is a potential biomarker for many diseases as it plays important roles in inflammatory disorders and autoimmune diseases, and has been implicated in carcinogenesis and acute myocardial infarction.
The LEGEND MAX™ Human MRP14 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human MRP14 capture antibody. The Detection Antibody is a biotinylated form of the same mouse monoclonal anti-human MRP14 antibody. This kit has been analytically validated to accurately measure MRP14 homodimers in serum and plasma samples with ready-to-use reagents.Product Details
- Kit Contents
- Anti-Human MRP14 Pre-coated 96-well Strip Microplate
- Human MRP14 Dectection Antibody
- Human MRP14 Standard
- Matrix B
- Assay Buffer B
- Assay Diluent E
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Application Notes
This kit detects MRP14 with better accuracy than other kits on the market with the same diversity of valid sample types. The spiked recombinant protein recovery of this kit is 101%, while an equivalent kit from a major competitor reported 93%. In addition this kit is offered at a very competitive price when compared to equivalent ELISA products currently offered by major competitors.
- 63.1 pg/mL ± 34.6 pg/mL (mean ± 2 SD; n=9)
- Standard Range
- 313-20,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.