- Clone
- J099B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histidine Tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
A 96 well plate is coated with 100 µL of a His-tagged recombinant protein at 100 ng/mL. The coated protein is detected with HRP-conjugated anti-His tag antibody at concentration ranged from 1 to 0.008 µg/mL. -
A His-tagged recombinant protein is diluted in cell-culture medium. The diluted protein is loaded to 12% SDS PAGE gel in amount of 40, 20, and 10 ng. After electrophoresis, the His-tagged recombinant protein is detected with 0.3 µg/mL of the HRP-conjugated anti-His tag monoclonal antibody.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 652503 | 25 µg | £65.00 | ||||
| 652504 | 100 µg | £239.00 | ||||
This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).
Product DetailsProduct Details
- Reactivity
- All species (6x His tag epitope)
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
- Application
-
ELISA - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 µg per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.
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Application References
(PubMed link indicates BioLegend citation) -
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Product Citations
- RRID
-
AB_2734520 (BioLegend Cat. No. 652503)
AB_2563555 (BioLegend Cat. No. 652504)
Antigen Details
- Function
- Recombinant poly-histidine tag detection
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about His Tag on UniProt.org
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