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HRP anti-Alpha-I Spectrin Antibody

Pricing & Availability
Clone
17C7 (See other available formats)
Regulatory Status
RUO
Other Names
EL2, SPTA1, Spectrin alpha chain, erythrocyte, alpha-I spectrin, erythroid alpha-spectrin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
17C7_HRP_AlphaISpectrin_WB_110918
Western blot of HRP anti-Alpha-I Spectrin antibody (clone 17C7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • 17C7_HRP_AlphaISpectrin_WB_110918
    Western blot of HRP anti-Alpha-I Spectrin antibody (clone 17C7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
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803105 25 µg £105
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803106 100 µg £221
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Description

Spectrin is an intracellular cytoskeletal protein that provides structure to eukaryotic cells and helps maintain plasma membrane integrity. Alpha-1 Spectrin, encoded by the SPTA1 gene, is found primarily in erythrocytes and in the cerebellum.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Clone 17C7 is developed against the alpha I spectrin SH3 domain (Xu et al., 2000)2.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in Western Blotting (WB). Additional reported applications include immunocytochemistry1, 2.

Clone 17C7 is a specific marker for human, rat and mouse alpha I spectrin. It reacts with erythroid spectrin in red blood cells and in cerebellum. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 280 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes1, 2.

This antibody was developed against a recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

Application References

(PubMed link indicates BioLegend citation)
  1. Xu J, et al. 2001. Brain Res. 898(1):171-7. (WB, ICC)
  2. Xu J, et al. 2000. J. Cell Sci.113:3805-14. (WB, ICC)
Product Citations
  1. Yang JT, et al. 2018. Neurosci Bull. 34:736. PubMed
RRID
AB_2783375 (BioLegend Cat. No. 803105)
AB_2783376 (BioLegend Cat. No. 803106)

Antigen Details

Structure
Expected MW: 250 kD
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. De Matteis MA, Morrow JS. 2000. J. Cell Sci. 113:2331-43.
  2. Bennett V, Gilligan DM. 1993. Annu. Rev. Cell Biol. 9:27-66.
Gene ID
6708 View all products for this Gene ID 20739 View all products for this Gene ID 289257 View all products for this Gene ID
UniProt
View information about Alpha-I Spectrin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/12/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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