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GoInVivo™ Purified anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
RMT3-23 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMT3-23_GoInVivo_CD366_Tim-3_Antibody_080916
Mouse Tim-3 binds human Galectin-9 in a dose dependent manner (purple circles). When mouse Tim-3 at a fixed concentration (2 µg/ml) was pre-incubated with increasing concentrations of anti-mouse Tim-3 (clone RMT3-23, purple squares) prior to adding to the wells containing immobilized human Galectin-9, clone RMT3-23 inhibited Tim-3/Galectin-9 interaction in a dose dependent manner.
  • RMT3-23_GoInVivo_CD366_Tim-3_Antibody_080916
    Mouse Tim-3 binds human Galectin-9 in a dose dependent manner (purple circles). When mouse Tim-3 at a fixed concentration (2 µg/ml) was pre-incubated with increasing concentrations of anti-mouse Tim-3 (clone RMT3-23, purple squares) prior to adding to the wells containing immobilized human Galectin-9, clone RMT3-23 inhibited Tim-3/Galectin-9 interaction in a dose dependent manner.
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119712 5 mg £490
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119713 25 mg £1355
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119714 50 mg £2107
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119709 100 mg £3650
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119710 500 mg £12603
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119711 1 g £19563
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
  2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
RRID
AB_2616635 (BioLegend Cat. No. 119712)
AB_2616636 (BioLegend Cat. No. 119713)
AB_2616637 (BioLegend Cat. No. 119714)
AB_2616626 (BioLegend Cat. No. 119709)
AB_2616627 (BioLegend Cat. No. 119710)
AB_2616628 (BioLegend Cat. No. 119711)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

Function
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Cell Type
Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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