FITC anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
h1.2f3
PMA-stimulated (6 hours) splenocytes stained with H1.2F3 FITC
  • h1.2f3
    PMA-stimulated (6 hours) splenocytes stained with H1.2F3 FITC
See FITC spectral data
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104505 50 µg £48.00
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104506 500 µg £171.00
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Riva A, et al. 2017. PLoS One.. 10.1371/journal.pone.0181964. PubMed
  2. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  3. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  4. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  5. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  6. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  7. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  8. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  9. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  10. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  11. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  12. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  13. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  14. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  15. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  16. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  17. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  18. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  19. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  20. Brummer G, et al. 2020. Oncogene. 39:2275. PubMed
  21. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  22. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  23. Klezovich-Bénard M, et al. 2012. PLoS One. 8:e1002481. PubMed
  24. Stanford S, et al. 2012. Proc Natl Acad Sci U S A. 109:13972. PubMed
  25. Tao F, et al. 2013. Biochem Pharmacol. 85:798. PubMed
  26. Haile S, et al. 2014. Cancer Immunol Res. 2:610. PubMed
  27. White C, et al. 2015. J Immunol. 194:697. PubMed
  28. XS R, et al. 2015. Diabetes. 64 90. PubMed
  29. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  30. Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed
  31. Han J, et al. 2015. Cancer Res . 75: 5273 - 5282. PubMed
  32. Cordero-Reyes A, et al. 2016. J Am Heart Assoc. 5: 002484. PubMed
  33. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  34. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  35. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  36. Uhde A, et al. 2016. PLoS One. 11: 0161883. PubMed
  37. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
  38. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  39. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
RRID
AB_313108 (BioLegend Cat. No. 104505)
AB_313109 (BioLegend Cat. No. 104506)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/18/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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