FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
109805 50 µg £31.00
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109806 500 µg £72.00
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  2. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  3. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  4. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  5. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  6. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  7. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  8. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  9. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  10. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  11. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  12. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  13. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  14. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  15. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  16. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  17. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  18. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  19. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  20. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  21. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  22. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  23. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  24. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  25. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  26. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  27. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  28. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  29. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  30. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  31. Wan X, et al. 2018. Nature. 560:107. PubMed
  32. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  33. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  34. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  35. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  36. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  37. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  38. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  39. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  40. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  41. Burman A, et al. 2007. Blood. 110:1064. PubMed
  42. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  43. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  44. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  45. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  46. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  47. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  48. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  49. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  50. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  51. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  52. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  53. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  54. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  55. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  56. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  57. Li J, et al. 2020. Elife. 9:00. PubMed
  58. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  59. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  60. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  61. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  62. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  63. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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